Glycine receptor antibody - 146 011BT

An inhibitory receptor in the nervous system

Mouse monoclonal purified IgG

Cat. No.: 146 011BT

Amount: 100 µg

Price: $470.00

Cat. No. 146 011BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) IP: yes ICC: not tested yet IHC: 1 : 250 up to 1 : 500 (see remarks) gallery IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	mAb4a
Subtype	IgG1 (κ light chain)
Immunogen	Nativ Protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop	AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity	Reacts with: human (P23415, P23416, P48167), rat (P07727, P22771, P20781), mouse (Q64018, Q7TNC8, P48168), pig, zebrafish. Other species not tested yet.
Specificity	Specific for all glycine receptor subunits.
Remarks	IHC: Antigen retrieval with methanol/acetic acid is required. For details see Dumoulin A, Triller A & Dieudonné S (2001).
Data sheet	Data Sheet 146_011bt Safety Data Sheet (SDS)

References for Glycine receptor - 146 011BT

Agility training enhances motor temporal precision by reweighting spinal phase-locked commissural inhibition.
Liu W, Zhao Y, Yang J, Liu Z, Ren W, Wang Z, Zhao Y, Guan NN, Song J
Current biology : CB (2026) 3612: 3032-3042.e5. 146 011BT IHC; tested species: zebrafish

Persisting Region-Specific Microglia Activation and Synaptic Imbalance During Remission in a DSS-Induced Colitis Model.
Morton L, Villafuerte LE, Garza AP, Lindemann N, Wider-Eberspächer E, Bruder D, Dunay IR
Glia (2026) 7411: e70208. 146 011BT FACS; tested species: mouse

Cat. No.: 146 011BT

Amount: 100 µg

Price: $470.00

IHC
(1 img)

Background

The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.

1 ) Bechade C et al., Eur. J. Neurosci. (1996)
2 ) Becker CM et al., J. Neurosci. (1986)
3 ) Altschuler RA et al., Brain Res. (1986)
4 ) Triller A et al., J. Cell Biol. (1985)
5 ) Graham D et al., Biochemistry (1985)

Protocols

IHC

Standard Condition

Other products for this target