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Home > Products > 146 011BT

Glycine receptor antibody - 146 011BT

An inhibitory receptor in the nervous system
Mouse monoclonal purified IgG
Cat. No.: 146 011BT
Amount: 100 µg
Price: $465.00
Cat. No. 146 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining)
IP: yes
ICC: yes
IHC: 1 : 250 up to 1 : 500 (see remarks) gallery  
IHC-P: not tested yet
ELISA: yes (see remarks)
Label biotin
Clone mAb4a
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 457 from rat Glycine receptor α1 (UniProt Id: P07727)
Epitop Epitop: AA 96 to 105 from rat Glycine receptor α1 (UniProt Id: P07727)
Reactivity Reacts with: human (P23415, P23416, P48167), rat (P07727, P22771, P20781), mouse (Q64018, Q7TNC8, P48168), pig, zebrafish.
Other species not tested yet.
Specificity Specific for all glycine receptor subunits.
Remarks

IHC: Tissue sections require additional antigen retrieval with methanol/acetic acid prior to antibody incubation. For details see Dumoulin A, Triller A & Dieudonné S (2001).
ELISA: The ELISA-protocol for membrane proteins is recommended.
Data sheet 146_011bt.pdf
Cat. No.: 146 011BT
Amount: 100 µg
Price: $465.00
IHC
Background
The inhibitory glycine receptor (GlyR) is a member of the ligand-gated ion channel superfamily of neurotransmitter receptors. It is an oligomeric protein composed of homologous subunits (α 1-4 and β) with four transmembrane segments (M1-M4) each.
It shows a widespread expression profile in brain. Several isoforms and splice variants with distinct pharmacology have been discovered so far.
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