|Cat. No. 143 103||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.|
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not tested yet
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: not tested yet
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 655 to 670 from rat ERC2 (UniProt Id: Q8K3M6)|
Reacts with: rat (Q8K3M6), mouse (Q6PH08), hamster.
No signal: zebrafish.
Other species not tested yet.
|Specificity||Specific for ERC 2. K.O. PubMed: 27422015|
|Matching control protein/peptide||143-1P|
ELKS, also referred to as ERCs (ERC 1 and ERC 2) and CAST, are related proteins which share an identical C-terminal sequence. They interact with the conserved RIM PDZ domain via an unusual PDZ binding motif. Two splice variants of ERC 1 (1a and 1b) have been described. ERC 1b (CAST 2a) binds to RIM and is expressed exclusively in the brain. ERC 1a is a ubiquitously expressed cytosolic protein. ERC 2 (CAST 1) is only expressed as a single RIM binding variant.
All ERCs have been shown to interact with Rab 6, a protein involved in membrane trafficking at the Golgi complex. The function of these proteins has not been determined yet. They may link Rab 6 mediated non-neuronal membrane traffic at the Golgi complex to neuronal membrane traffic at the active zone executed via RIMs.