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Piccolo antibody - 142 002 K.O.

Piccolo is a large multi-domain protein of the presynaptic active zone
Rabbit polyclonal antiserum
Cat. No.: 142 002
Amount: 200 µl
Price: $355.00
Cat. No. 142 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (ECL detection) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: yes gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 4439 to 4776 from rat Piccolo (UniProt Id: Q9JKS6)
Reactivity Reacts with: rat (Q9JKS6), mouse (Q9QYX7).
Other species not tested yet.
Specificity K.O. validated PubMed: 27537483
Matching control protein/peptide 142-0P
Remarks

WB: Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

This antibody detects an additional band of ~65 kDa.

Data sheet 142_002.pdf

References for Piccolo - 142 002

ADAM10 hyperactivation acts on piccolo to deplete synaptic vesicle stores in Huntington's disease.
Cozzolino F, Vezzoli E, Cheroni C, Besusso D, Conforti P, Valenza M, Iacobucci I, Monaco V, Birolini G, Bombaci M, Falqui A, et al.
Human molecular genetics (2021) : . 142 002 WB, IP; tested species: mouse
Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 142 002 WB; KO verified; tested species: rat
Presynaptic calcium channels and α3-integrins are complexed with synaptic cleft laminins, cytoskeletal elements and active zone components.
Carlson SS, Valdez G, Sanes JR
Journal of neurochemistry (2010) 1153: 654-66. 142 002 WB; tested species: mouse
APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle.
Szodorai A, Kuan YH, Hunzelmann S, Engel U, Sakane A, Sasaki T, Takai Y, Kirsch J, Müller U, Beyreuther K, Brady S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2009) 2946: 14534-44. 142 002 WB
Molecular dynamics of photoreceptor synapse formation in the developing chick retina.
Wahlin KJ, Moreira EF, Huang H, Yu N, Adler R
The Journal of comparative neurology (2008) 5065: 822-37. 142 002 WB, IHC
ADAM10 hyperactivation acts on piccolo to deplete synaptic vesicle stores in Huntington's disease.
Cozzolino F, Vezzoli E, Cheroni C, Besusso D, Conforti P, Valenza M, Iacobucci I, Monaco V, Birolini G, Bombaci M, Falqui A, et al.
Human molecular genetics (2021) : . 142 002 WB, IP; tested species: mouse
The metabolite p-cresol impairs dendritic development, synaptogenesis, and synapse function in hippocampal neurons: Implications for autism spectrum disorder.
Guzmán-Salas S, Weber A, Malci A, Lin X, Herrera-Molina R, Cerpa W, Dorador C, Signorelli J, Zamorano P
Journal of neurochemistry (2022) 1614: 335-349. 142 002 ICC; tested species: rat
Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins.
Uemura T, Suzuki-Kouyama E, Kawase S, Kurihara T, Yasumura M, Yoshida T, Fukai S, Yamazaki M, Fei P, Abe M, Watanabe M, et al.
Cell reports (2022) 391: 110624. 142 002 ICC; tested species: mouse
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 142 002 ICC; tested species: mouse
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 142 002 ICC, IHC; tested species: human,mouse
Identification of Potential Interacting Proteins With the Extracellular Loops of the Neuronal Glycoprotein M6a by TMT/MS.
Aparicio GI, Formoso K, León A, Frasch AC, Scorticati C
Frontiers in synaptic neuroscience (2020) 12: 28. 142 002 ICC; tested species: rat
Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface.
Funahashi J, Tanaka H, Hirano T
Frontiers in cellular neuroscience (2018) 12: 140. 142 002 ICC; tested species: rat
Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 142 002 ICC
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 142 002 ICC; KO verified
Rapid structural alterations of the active zone lead to sustained changes in neurotransmitter release.
Matz J, Gilyan A, Kolar A, McCarvill T, Krueger SR
Proceedings of the National Academy of Sciences of the United States of America (2010) 10719: 8836-41. 142 002 ICC; tested species: rat
Localization of the active zone proteins CAST, ELKS, and Piccolo at neuromuscular junctions.
Tokoro T, Higa S, Deguchi-Tawarada M, Inoue E, Kitajima I, Ohtsuka T
Neuroreport (2007) 184: 313-6. 142 002 ICC
Assembly of active zone precursor vesicles: obligatory trafficking of presynaptic cytomatrix proteins Bassoon and Piccolo via a trans-Golgi compartment.
Dresbach T, Torres V, Wittenmayer N, Altrock WD, Zamorano P, Zuschratter W, Nawrotzki R, Ziv NE, Garner CC, Gundelfinger ED
The Journal of biological chemistry (2006) 2819: 6038-47. 142 002 ICC
Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification.
Juranek J, Mukherjee K, Rickmann M, Martens H, Calka J, Südhof TC, Jahn R
The European journal of neuroscience (2006) 2411: 3043-52. 142 002 ICC
Molecular profiling reveals synaptic release machinery in Merkel cells.
Haeberle H, Fujiwara M, Chuang J, Medina MM, Panditrao MV, Bechstedt S, Howard J, Lumpkin EA
Proceedings of the National Academy of Sciences of the United States of America (2004) 10140: 14503-8. 142 002 ICC
Coordinated bi-directional trafficking of synaptic vesicle and active zone proteins in peripheral nerves.
Juranek JK, Mukherjee K, Jahn R, Li JY
Biochemical and biophysical research communications (2021) 559: 92-98. 142 002 IHC; tested species: rat
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 142 002 ICC, IHC; tested species: human,mouse
Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation.
Oprişoreanu AM, Smith HL, Arya S, Webster R, Zhong Z, Wehner D, Cardozo MJ, Becker T, Talbot K, Becker CG
Cell reports (2019) 295: 1082-1098.e10. 142 002 IHC; tested species: mouse
Multiple roles of integrin-α3 at the neuromuscular junction.
Ross JA, Webster RG, Lechertier T, Reynolds LE, Turmaine M, Bencze M, Jamshidi Y, Cetin H, Muntoni F, Beeson D, Hodilvala-Dilke K, et al.
Journal of cell science (2017) 13010: 1772-1784. 142 002 IHC; tested species: mouse
Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice.
Nishimune H, Badawi Y, Mori S, Shigemoto K
Scientific reports (2016) 6: 27935. 142 002 IHC; tested species: mouse
Calcium channels link the muscle-derived synapse organizer laminin β2 to Bassoon and CAST/Erc2 to organize presynaptic active zones.
Chen J, Billings SE, Nishimune H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 312: 512-25. 142 002 IHC; tested species: mouse
Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys.
Ribic A, Zhang M, Schlumbohm C, Mätz-Rensing K, Uchanska-Ziegler B, Flügge G, Zhang W, Walter L, Fuchs E
Cellular and molecular neurobiology (2010) 306: 827-39. 142 002 IHC
Molecular dynamics of photoreceptor synapse formation in the developing chick retina.
Wahlin KJ, Moreira EF, Huang H, Yu N, Adler R
The Journal of comparative neurology (2008) 5065: 822-37. 142 002 WB, IHC
Cat. No.: 142 002
Amount: 200 µl
Price: $355.00
ADAM10 hyperactivation acts on piccolo to deplete synaptic vesicle stores in Huntington's disease.
Cozzolino F, Vezzoli E, Cheroni C, Besusso D, Conforti P, Valenza M, Iacobucci I, Monaco V, Birolini G, Bombaci M, Falqui A, et al.
Human molecular genetics (2021) : . 142 002 WB, IP; tested species: mouse
Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 142 002 WB; KO verified; tested species: rat
Presynaptic calcium channels and α3-integrins are complexed with synaptic cleft laminins, cytoskeletal elements and active zone components.
Carlson SS, Valdez G, Sanes JR
Journal of neurochemistry (2010) 1153: 654-66. 142 002 WB; tested species: mouse
APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle.
Szodorai A, Kuan YH, Hunzelmann S, Engel U, Sakane A, Sasaki T, Takai Y, Kirsch J, Müller U, Beyreuther K, Brady S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2009) 2946: 14534-44. 142 002 WB
Molecular dynamics of photoreceptor synapse formation in the developing chick retina.
Wahlin KJ, Moreira EF, Huang H, Yu N, Adler R
The Journal of comparative neurology (2008) 5065: 822-37. 142 002 WB, IHC
ADAM10 hyperactivation acts on piccolo to deplete synaptic vesicle stores in Huntington's disease.
Cozzolino F, Vezzoli E, Cheroni C, Besusso D, Conforti P, Valenza M, Iacobucci I, Monaco V, Birolini G, Bombaci M, Falqui A, et al.
Human molecular genetics (2021) : . 142 002 WB, IP; tested species: mouse
The metabolite p-cresol impairs dendritic development, synaptogenesis, and synapse function in hippocampal neurons: Implications for autism spectrum disorder.
Guzmán-Salas S, Weber A, Malci A, Lin X, Herrera-Molina R, Cerpa W, Dorador C, Signorelli J, Zamorano P
Journal of neurochemistry (2022) 1614: 335-349. 142 002 ICC; tested species: rat
Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins.
Uemura T, Suzuki-Kouyama E, Kawase S, Kurihara T, Yasumura M, Yoshida T, Fukai S, Yamazaki M, Fei P, Abe M, Watanabe M, et al.
Cell reports (2022) 391: 110624. 142 002 ICC; tested species: mouse
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 142 002 ICC; tested species: mouse
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 142 002 ICC, IHC; tested species: human,mouse
Identification of Potential Interacting Proteins With the Extracellular Loops of the Neuronal Glycoprotein M6a by TMT/MS.
Aparicio GI, Formoso K, León A, Frasch AC, Scorticati C
Frontiers in synaptic neuroscience (2020) 12: 28. 142 002 ICC; tested species: rat
Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface.
Funahashi J, Tanaka H, Hirano T
Frontiers in cellular neuroscience (2018) 12: 140. 142 002 ICC; tested species: rat
Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 142 002 ICC
Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking.
Wang SSH, Held RG, Wong MY, Liu C, Karakhanyan A, Kaeser PS
Neuron (2016) 914: 777-791. 142 002 ICC; KO verified
Rapid structural alterations of the active zone lead to sustained changes in neurotransmitter release.
Matz J, Gilyan A, Kolar A, McCarvill T, Krueger SR
Proceedings of the National Academy of Sciences of the United States of America (2010) 10719: 8836-41. 142 002 ICC; tested species: rat
Localization of the active zone proteins CAST, ELKS, and Piccolo at neuromuscular junctions.
Tokoro T, Higa S, Deguchi-Tawarada M, Inoue E, Kitajima I, Ohtsuka T
Neuroreport (2007) 184: 313-6. 142 002 ICC
Assembly of active zone precursor vesicles: obligatory trafficking of presynaptic cytomatrix proteins Bassoon and Piccolo via a trans-Golgi compartment.
Dresbach T, Torres V, Wittenmayer N, Altrock WD, Zamorano P, Zuschratter W, Nawrotzki R, Ziv NE, Garner CC, Gundelfinger ED
The Journal of biological chemistry (2006) 2819: 6038-47. 142 002 ICC
Differential expression of active zone proteins in neuromuscular junctions suggests functional diversification.
Juranek J, Mukherjee K, Rickmann M, Martens H, Calka J, Südhof TC, Jahn R
The European journal of neuroscience (2006) 2411: 3043-52. 142 002 ICC
Molecular profiling reveals synaptic release machinery in Merkel cells.
Haeberle H, Fujiwara M, Chuang J, Medina MM, Panditrao MV, Bechstedt S, Howard J, Lumpkin EA
Proceedings of the National Academy of Sciences of the United States of America (2004) 10140: 14503-8. 142 002 ICC
Coordinated bi-directional trafficking of synaptic vesicle and active zone proteins in peripheral nerves.
Juranek JK, Mukherjee K, Jahn R, Li JY
Biochemical and biophysical research communications (2021) 559: 92-98. 142 002 IHC; tested species: rat
No symphony without bassoon and piccolo: changes in synaptic active zone proteins in Huntington's disease.
Huang TT, Smith R, Bacos K, Song DY, Faull RM, Waldvogel HJ, Li JY
Acta neuropathologica communications (2020) 81: 77. 142 002 ICC, IHC; tested species: human,mouse
Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation.
Oprişoreanu AM, Smith HL, Arya S, Webster R, Zhong Z, Wehner D, Cardozo MJ, Becker T, Talbot K, Becker CG
Cell reports (2019) 295: 1082-1098.e10. 142 002 IHC; tested species: mouse
Multiple roles of integrin-α3 at the neuromuscular junction.
Ross JA, Webster RG, Lechertier T, Reynolds LE, Turmaine M, Bencze M, Jamshidi Y, Cetin H, Muntoni F, Beeson D, Hodilvala-Dilke K, et al.
Journal of cell science (2017) 13010: 1772-1784. 142 002 IHC; tested species: mouse
Dual-color STED microscopy reveals a sandwich structure of Bassoon and Piccolo in active zones of adult and aged mice.
Nishimune H, Badawi Y, Mori S, Shigemoto K
Scientific reports (2016) 6: 27935. 142 002 IHC; tested species: mouse
Calcium channels link the muscle-derived synapse organizer laminin β2 to Bassoon and CAST/Erc2 to organize presynaptic active zones.
Chen J, Billings SE, Nishimune H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 312: 512-25. 142 002 IHC; tested species: mouse
Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys.
Ribic A, Zhang M, Schlumbohm C, Mätz-Rensing K, Uchanska-Ziegler B, Flügge G, Zhang W, Walter L, Fuchs E
Cellular and molecular neurobiology (2010) 306: 827-39. 142 002 IHC
Molecular dynamics of photoreceptor synapse formation in the developing chick retina.
Wahlin KJ, Moreira EF, Huang H, Yu N, Adler R
The Journal of comparative neurology (2008) 5065: 822-37. 142 002 WB, IHC
Background

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Piccolo is supposed to be a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1.