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RIM1/2 antibody Zn-finger domain - 140 205

RIMs are Rab3 effector proteins of the cytomatrix at the active zone
Guinea pig polyclonal purified antibody
Cat. No.: 140 205
Amount: 50 µg
Price: $460.00
Cat. No. 140 205 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
ExM: 1 : 200 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.

Immunogen Recombinant protein corresponding to AA 1 to 466 from rat Rim2 (UniProt Id: Q9JIS1)
Reactivity Reacts with: rat (Q9JIS1), mouse (Q9EQZ7).
Other species not tested yet.
Specificity RIM 2 including splice variants, cross reacts to RIM 1.
Remarks

ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)

Data sheet 140_205.pdf

References for RIM1/2 - 140 205

Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus.
Lloyd BA, Han Y, Roth R, Zhang B, Aoto J
Nature communications (2023) 141: 4706. 140 205 WB; tested species: mouse
Functional specificity of liquid-liquid phase separation at the synapse.
Guzikowski NJ, Kavalali ET
Nature communications (2024) 151: 10103. 140 205 ICC; tested species: rat
Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses.
Yang X, Le Corronc H, Legendre P, Triller A, Specht CG
EMBO reports (2021) : e52154. 140 205 ICC; tested species: rat
Excitatory and inhibitory synapses show a tight subcellular correlation that weakens over development.
Horton S, Mastrolia V, Jackson RE, Kemlo S, Pereira Machado PM, Carbajal MA, Hindges R, Fleck RA, Aguiar P, Neves G, Burrone J, et al.
Cell reports (2024) 437: 114361. 140 205 IHC; tested species: mouse
Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 140 205 IHC; tested species: mouse
Cat. No.: 140 205
Amount: 50 µg
Price: $460.00
Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus.
Lloyd BA, Han Y, Roth R, Zhang B, Aoto J
Nature communications (2023) 141: 4706. 140 205 WB; tested species: mouse
Functional specificity of liquid-liquid phase separation at the synapse.
Guzikowski NJ, Kavalali ET
Nature communications (2024) 151: 10103. 140 205 ICC; tested species: rat
Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses.
Yang X, Le Corronc H, Legendre P, Triller A, Specht CG
EMBO reports (2021) : e52154. 140 205 ICC; tested species: rat
Excitatory and inhibitory synapses show a tight subcellular correlation that weakens over development.
Horton S, Mastrolia V, Jackson RE, Kemlo S, Pereira Machado PM, Carbajal MA, Hindges R, Fleck RA, Aguiar P, Neves G, Burrone J, et al.
Cell reports (2024) 437: 114361. 140 205 IHC; tested species: mouse
Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 140 205 IHC; tested species: mouse
Background

RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.
RIM 2β consists of a specific N-terminus, the central PDZ domain and the C-terminal C2 domains. The mRNA for RIM 2β is transcribed from an internal promoter of the RIM 2α gene.
Shorter variants of RIM 2 which comprise only the C-terminal C2B domain and some flanking regions are referred to as NIM 2 / RIM 2γ and NIM 3 / RIM 3γ.