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RIM1 antibody PDZ domain - 140 005

RIMs are Rab3 effector proteins of the cytomatrix at the active zone
Guinea pig polyclonal purified antibody
Cat. No.: 140 005
Amount: 50 µg
Price: $460.00
Cat. No. 140 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not recommended
Immunogen Recombinant protein corresponding to AA 596 to 705 from rat Rim1 (UniProt Id: Q9JIR4)
Reactivity Reacts with: rat (Q9JIR4), mouse (Q99NE5).
Other species not tested yet.
Specificity Specific for RIM 1 with weak cross reactivity to RIM 2.
Remarks

IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).

Data sheet 140_005.pdf

References for RIM1 - 140 005

Correction of amygdalar dysfunction in a rat model of fragile X syndrome.
Fernandes G, Mishra PK, Nawaz MS, Donlin-Asp PG, Rahman MM, Hazra A, Kedia S, Kayenaat A, Songara D, Wyllie DJA, Schuman EM, et al.
Cell reports (2021) 372: 109805. 140 005 IHC; tested species: rat
Cat. No.: 140 005
Amount: 50 µg
Price: $460.00
Correction of amygdalar dysfunction in a rat model of fragile X syndrome.
Fernandes G, Mishra PK, Nawaz MS, Donlin-Asp PG, Rahman MM, Hazra A, Kedia S, Kayenaat A, Songara D, Wyllie DJA, Schuman EM, et al.
Cell reports (2021) 372: 109805. 140 005 IHC; tested species: rat
Background

RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.