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VGLUT 1 antibody - 135 308

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Rabbit monoclonal recombinant IgG
Cat. No.: 135 308
Amount: 50 µg
Price: $415.00
Cat. No. 135 308 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 1000 gallery  
Clone Rb68B7
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT 1. (UniProt Id: Q62634)
Epitop Epitop: AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity Specific for VGLUT 1 K.O.
Matching control protein/peptide 135-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 68B7. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: This antibody is highly recommended for Western blot experiments. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_308.pdf

References for VGLUT 1 - 135 308

Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale Study.
Jablonski J, Hoffmann L, Blümcke I, Fejtová A, Uebe S, Ekici AB, Gnatkovsky V, Kobow K
Cells (2021) 1011: . 135 308 ICC; tested species: rat
Glutamate released by Cajal-Retzius cells impacts specific hippocampal circuits and behaviors.
Anstötz M, Lee SK, Maccaferri G
Cell reports (2022) 397: 110822. 135 308 IHC; tested species: mouse
Dorsal and ventral striatal neuronal subpopulations differentially disrupt male mouse copulatory behavior.
Detraux B, Vilella A, De Groote A, Schiffmann SN, Zoli M, de Kerchove d'Exaerde A
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology (2021) 49: 23-37. 135 308 IHC; tested species: mouse
Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Liang L, Zeng T, Zhao Y, Lu R, Guo B, Xie R, Tang W, Zhang L, Mao Z, Yang X, Wu S, et al.
Journal of pineal research (2021) 714: e12771. 135 308 IHC; tested species: mouse
Cat. No.: 135 308
Amount: 50 µg
Price: $415.00
Experimental Epileptogenesis in a Cell Culture Model of Primary Neurons from Rat Brain: A Temporal Multi-Scale Study.
Jablonski J, Hoffmann L, Blümcke I, Fejtová A, Uebe S, Ekici AB, Gnatkovsky V, Kobow K
Cells (2021) 1011: . 135 308 ICC; tested species: rat
Glutamate released by Cajal-Retzius cells impacts specific hippocampal circuits and behaviors.
Anstötz M, Lee SK, Maccaferri G
Cell reports (2022) 397: 110822. 135 308 IHC; tested species: mouse
Dorsal and ventral striatal neuronal subpopulations differentially disrupt male mouse copulatory behavior.
Detraux B, Vilella A, De Groote A, Schiffmann SN, Zoli M, de Kerchove d'Exaerde A
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology (2021) 49: 23-37. 135 308 IHC; tested species: mouse
Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Liang L, Zeng T, Zhao Y, Lu R, Guo B, Xie R, Tang W, Zhang L, Mao Z, Yang X, Wu S, et al.
Journal of pineal research (2021) 714: e12771. 135 308 IHC; tested species: mouse
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.