Cat. No.: 135 011BT
Amount: 100 µg
Price:
$470.00
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| Cat. No. 135 011BT |
100 µg purified IgG, lyophilized, labeled with Biotin.
Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Label | biotin |
| Clone | 68B7 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Synthetic peptide corresponding to AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634) |
| Reactivity |
Reacts with: mouse (Q3TXX4), rat (Q62634), human (Q9P2U7). Other species not tested yet. |
| Specificity | K.O. validated |
| Matching control protein/peptide | 135-0P |
| Remarks |
WB: This antibody is highly recommended for Western blot experiments. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples |
| Data sheet | 135_011bt.pdf |
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The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.