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GFP antibody - 132 004

GFP is a widely used green fluorescent protein tag
Guinea pig polyclonal antiserum
Cat. No.: 132 004
Amount: 100 µl
Price: $365.00
Cat. No. 132 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
EM: yes
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants. This antibody still recognizes its antigen after heat mediated antigen retrieval. It does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Data sheet 132_004.pdf

References for GFP - 132 004

Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses.
Chiacchiaretta M, Latifi S, Bramini M, Fadda M, Fassio A, Benfenati F, Cesca F
Journal of cell science (2017) 1308: 1435-1449. 132 004 ICC; tested species: mouse
Immune response and pathogen invasion at the choroid plexus in the onset of cerebral toxoplasmosis.
Figueiredo CA, Steffen J, Morton L, Arumugam S, Liesenfeld O, Deli MA, Kröger A, Schüler T, Dunay IR
Journal of neuroinflammation (2022) 191: 17. 132 004 IHC; tested species: mouse
Impaired synaptic transmission in dorsal dentate gyrus increases impulsive alcohol seeking.
Nalberczak-Skóra M, Beroun A, Skonieczna E, Cały A, Ziółkowska M, Pagano R, Taheri P, Kalita K, Salamian A, Radwanska K
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2022) : . 132 004 IHC; tested species: mouse
The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects.
Lambert E, Saha O, Soares Landeira B, Melo de Farias AR, Hermant X, Carrier A, Pelletier A, Gadaut J, Davoine L, Dupont C, Amouyel P, et al.
Acta neuropathologica communications (2022) 101: 4. 132 004 IHC; tested species: drosophila
Optogenetic stimulation of striatal patches modifies habit formation and inhibits dopamine release.
Nadel JA, Pawelko SS, Scott JR, McLaughlin R, Fox M, Ghanem M, van der Merwe R, Hollon NG, Ramsson ES, Howard CD
Scientific reports (2021) 111: 19847. 132 004 IHC; tested species: mouse
Novel 3D analysis using optical tissue clearing documents the evolution of murine rapidly progressive glomerulonephritis.
Puelles VG, Fleck D, Ortz L, Papadouri S, Strieder T, Böhner AMC, van der Wolde JW, Vogt M, Saritas T, Kuppe C, Fuss A, et al.
Kidney international (2019) 962: 505-516. 132 004 IHC; tested species: mouse
An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.
Batiuk MY, de Vin F, Duqué SI, Li C, Saito T, Saido T, Fiers M, Belgard TG, Holt MG
The Journal of biological chemistry (2017) 29221: 8874-8891. 132 004 IHC; tested species: mouse
Postsynaptic gephyrin clustering controls the development of adult-born granule cells in the olfactory bulb.
Deprez F, Pallotto M, Vogt F, Grabiec M, Virtanen MA, Tyagarajan SK, Panzanelli P, Fritschy JM
The Journal of comparative neurology (2015) 52313: 1998-2016. 132 004 EM
Cat. No.: 132 004
Amount: 100 µl
Price: $365.00
Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses.
Chiacchiaretta M, Latifi S, Bramini M, Fadda M, Fassio A, Benfenati F, Cesca F
Journal of cell science (2017) 1308: 1435-1449. 132 004 ICC; tested species: mouse
Immune response and pathogen invasion at the choroid plexus in the onset of cerebral toxoplasmosis.
Figueiredo CA, Steffen J, Morton L, Arumugam S, Liesenfeld O, Deli MA, Kröger A, Schüler T, Dunay IR
Journal of neuroinflammation (2022) 191: 17. 132 004 IHC; tested species: mouse
Impaired synaptic transmission in dorsal dentate gyrus increases impulsive alcohol seeking.
Nalberczak-Skóra M, Beroun A, Skonieczna E, Cały A, Ziółkowska M, Pagano R, Taheri P, Kalita K, Salamian A, Radwanska K
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2022) : . 132 004 IHC; tested species: mouse
The Alzheimer susceptibility gene BIN1 induces isoform-dependent neurotoxicity through early endosome defects.
Lambert E, Saha O, Soares Landeira B, Melo de Farias AR, Hermant X, Carrier A, Pelletier A, Gadaut J, Davoine L, Dupont C, Amouyel P, et al.
Acta neuropathologica communications (2022) 101: 4. 132 004 IHC; tested species: drosophila
Optogenetic stimulation of striatal patches modifies habit formation and inhibits dopamine release.
Nadel JA, Pawelko SS, Scott JR, McLaughlin R, Fox M, Ghanem M, van der Merwe R, Hollon NG, Ramsson ES, Howard CD
Scientific reports (2021) 111: 19847. 132 004 IHC; tested species: mouse
Novel 3D analysis using optical tissue clearing documents the evolution of murine rapidly progressive glomerulonephritis.
Puelles VG, Fleck D, Ortz L, Papadouri S, Strieder T, Böhner AMC, van der Wolde JW, Vogt M, Saritas T, Kuppe C, Fuss A, et al.
Kidney international (2019) 962: 505-516. 132 004 IHC; tested species: mouse
An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.
Batiuk MY, de Vin F, Duqué SI, Li C, Saito T, Saido T, Fiers M, Belgard TG, Holt MG
The Journal of biological chemistry (2017) 29221: 8874-8891. 132 004 IHC; tested species: mouse
Postsynaptic gephyrin clustering controls the development of adult-born granule cells in the olfactory bulb.
Deprez F, Pallotto M, Vogt F, Grabiec M, Virtanen MA, Tyagarajan SK, Panzanelli P, Fritschy JM
The Journal of comparative neurology (2015) 52313: 1998-2016. 132 004 EM
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.