|Cat. No. 132 003CpH||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E
CypHer5E is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E can be detected with the filter sets for Cy5TM.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 100 up to 1 : 1000 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Immunogen||Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)|
|Specificity||Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.|
This antibody is suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.