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GFP antibody - 132 003CpH

GFP is a widely used green fluorescent protein tag
Polyclonal rabbit purified antibody
Cat. No.: 132 003CpH
Amount: 50 µg
Price: $475.00
Cat. No. 132 003CpH 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E

CypHer5E is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E can be detected with the filter sets for Cy5TM.

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Application Number US 2006-0051758 and related patents. Cy, CyDye, and CypHer are trademarks of GE-Healthcare Ltd. Purchase of reagents related to the CypHer technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from GE-Healthcare Bio-Science Corp., 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label CypHer5E
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Remarks

This antibody is suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.

Data sheet 132_003cph.pdf

References for GFP - 132 003CpH

How to scale down postsynaptic strength.
Tatavarty V, Sun Q, Turrigiano GG
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3332: 13179-89. 132 003CpH UPTAKE
Cat. No.: 132 003CpH
Quantity: 50 µg
Price: $475.00
How to scale down postsynaptic strength.
Tatavarty V, Sun Q, Turrigiano GG
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3332: 13179-89. 132 003CpH UPTAKE
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.
Protocols