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GFP antibody - 132 002

GFP is a widely used green fluorescent protein tag
Rabbit polyclonal antiserum
Cat. No.: 132 002
Amount: 200 µl
Price: $355.00
Cat. No. 132 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 20000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: yes
EM: yes
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Data sheet 132_002.pdf

References for GFP - 132 002

Adamtsl3 mediates DCC signaling to selectively promote GABAergic synapse function.
Cramer TML, Pinan-Lucarre B, Cavaccini A, Damilou A, Tsai YC, Bhat MA, Panzanelli P, Rama N, Mehlen P, Benke D, Karayannis T, et al.
Cell reports (2023) 428: 112947. 132 002 WB; tested species: mouse
Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants.
Zlatic SA, Werner E, Surapaneni V, Lee CE, Gokhale A, Singleton K, Duong D, Crocker A, Gentile K, Middleton F, Dalloul JM, et al.
Human molecular genetics (2023) : . 132 002 WB, IP; tested species: mouse
Pharmacological perturbation of CXCL1 signaling alleviates neuropathogenesis in a model of HEVA71 infection.
Gunaseelan S, Ariffin MZ, Khanna S, Ooi MH, Perera D, Chu JJH, Chua JJE
Nature communications (2022) 131: 890. 132 002 WB; tested species: rat
FEZ1 forms complexes with CRMP1 and DCC to regulate axon and dendrite development.
Chua JY, Ng SJ, Yagensky O, Wanker EE, Chua JJE
eNeuro (2021) : . 132 002 WB; tested species: rat
Phosphorylation on Ser 359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses.
Nakamura Y, Morrow DH, Nathanson AJ, Henley JM, Wilkinson KA, Moss SJ
The Journal of biological chemistry (2020) : . 132 002 WB; tested species: rat
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 132 002 WB; tested species: human
Adaptor protein-3 complex is required for Vangl2 trafficking and planar cell polarity of the inner ear.
Tower-Gilchrist C, Zlatic SA, Yu D, Chang Q, Wu H, Lin X, Faundez V, Chen P
Molecular biology of the cell (2019) : mbcE16080592. 132 002 WB; tested species: mouse
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 132 002 WB; tested species: mouse
The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting.
Akula AK, Zhang X, Viotti JS, Nestvogel D, Rhee JS, Ebrecht R, Reim K, Wouters F, Liepold T, Jahn O, Bogeski I, et al.
Frontiers in molecular neuroscience (2019) 12: 249. 132 002 WB, ICC; tested species: mouse
Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged γ-Aminobutyric Acid Receptor, Type A (GABAA), α2 Subunit Knock-in Mouse.
Nakamura Y, Morrow DH, Modgil A, Huyghe D, Deeb TZ, Lumb MJ, Davies PA, Moss SJ
The Journal of biological chemistry (2016) 29123: 12394-407. 132 002 WB
Mover is a homomeric phospho-protein present on synaptic vesicles.
Ahmed S, Wittenmayer N, Kremer T, Hoeber J, Kiran Akula A, Urlaub H, Islinger M, Kirsch J, Dean C, Dresbach T
PloS one (2013) 85: e63474. 132 002 WB
Neuronal palmitoyl acyl transferases exhibit distinct substrate specificity.
Huang K, Sanders S, Singaraja R, Orban P, Cijsouw T, Arstikaitis P, Yanai A, Hayden MR, El-Husseini A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2009) 238: 2605-15. 132 002 WB, ICC
SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery.
Zhu GD, Salazar G, Zlatic SA, Fiza B, Doucette MM, Heilman CJ, Levey AI, Faundez V, L'hernault SW
Molecular biology of the cell (2009) 204: 1223-40. 132 002 WB
Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants.
Zlatic SA, Werner E, Surapaneni V, Lee CE, Gokhale A, Singleton K, Duong D, Crocker A, Gentile K, Middleton F, Dalloul JM, et al.
Human molecular genetics (2023) : . 132 002 WB, IP; tested species: mouse
CLASP2 Links Reelin to the Cytoskeleton during Neocortical Development.
Dillon GM, Tyler WA, Omuro KC, Kambouris J, Tyminski C, Henry S, Haydar TF, Beffert U, Ho A
Neuron (2017) 936: 1344-1358.e5. 132 002 IP
Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity.
Rotmann A, Strand D, Martiné U, Closs EI
The Journal of biological chemistry (2004) 27952: 54185-92. 132 002 IP
The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting.
Akula AK, Zhang X, Viotti JS, Nestvogel D, Rhee JS, Ebrecht R, Reim K, Wouters F, Liepold T, Jahn O, Bogeski I, et al.
Frontiers in molecular neuroscience (2019) 12: 249. 132 002 WB, ICC; tested species: mouse
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 132 002 ICC
Neuronal palmitoyl acyl transferases exhibit distinct substrate specificity.
Huang K, Sanders S, Singaraja R, Orban P, Cijsouw T, Arstikaitis P, Yanai A, Hayden MR, El-Husseini A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2009) 238: 2605-15. 132 002 WB, ICC
Control of the postsynaptic membrane viscosity.
Renner M, Choquet D, Triller A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2009) 299: 2926-37. 132 002 ICC; tested species: rat
Activity-dependent ubiquitination of GABA(A) receptors regulates their accumulation at synaptic sites.
Saliba RS, Michels G, Jacob TC, Pangalos MN, Moss SJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2007) 2748: 13341-51. 132 002 ICC
Gephyrin regulates the cell surface dynamics of synaptic GABAA receptors.
Jacob TC, Bogdanov YD, Magnus C, Saliba RS, Kittler JT, Haydon PG, Moss SJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2545: 10469-78. 132 002 ICC
Presence of ethanol-sensitive and ethanol-insensitive glycine receptors in the ventral tegmental area and prefrontal cortex in mice.
Araya A, Gallegos S, Viveros R, San Martin L, Muñoz B, Harvey RJ, Zeilhofer HU, Aguayo LG
British journal of pharmacology (2021) 17823: 4691-4707. 132 002 IHC; tested species: mouse
Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content.
Hartwig C, Méndez GM, Bhattacharjee S, Vrailas-Mortimer AD, Zlatic SA, Freeman AAH, Gokhale A, Concilli M, Werner E, Sapp Savas C, Rudin-Rush S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 412: 215-233. 132 002 IHC; tested species: drosophila
Temporal Dynamics and Neuronal Specificity of Grin3a Expression in the Mouse Forebrain.
Murillo A, Navarro AI, Puelles E, Zhang Y, Petros TJ, Pérez-Otaño I
Cerebral cortex (New York, N.Y. : 1991) (2020) : . 132 002 IHC; tested species: mouse
Semaphorin 2b Regulates Sleep-Circuit Formation in the Drosophila Central Brain.
Xie X, Tabuchi M, Corver A, Duan G, Wu MN, Kolodkin AL
Neuron (2019) 1042: 322-337.e14. 132 002 IHC
PBX transcription factors drive pulmonary vascular adaptation to birth.
McCulley DJ, Wienhold MD, Hines EA, Hacker TA, Rogers A, Pewowaruk RJ, Zewdu R, Chesler NC, Selleri L, Sun X
The Journal of clinical investigation (2018) 1282: 655-667. 132 002 IHC, IHC-P; tested species: mouse
Widespread transduction of astrocytes and neurons in the mouse central nervous system after systemic delivery of a self-complementary AAV-PHP.B vector.
Rincon MY, de Vin F, Duqué SI, Fripont S, Castaldo SA, Bouhuijzen-Wenger J, Holt MG
Gene therapy (2018) : . 132 002 IHC; tested species: mouse
An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.
Batiuk MY, de Vin F, Duqué SI, Li C, Saito T, Saido T, Fiers M, Belgard TG, Holt MG
The Journal of biological chemistry (2017) 29221: 8874-8891. 132 002 IHC; tested species: mouse
Morphological study of a connexin 43-GFP reporter mouse highlights glial heterogeneity, amacrine cells, and olfactory ensheathing cells.
Theofilas P, Steinhäuser C, Theis M, Derouiche A
Journal of neuroscience research (2017) 9511: 2182-2194. 132 002 IHC; tested species: mouse
CXC chemokine receptor 7 (CXCR7) regulates CXCR4 protein expression and capillary tuft development in mouse kidney.
Haege S, Einer C, Thiele S, Mueller W, Nietzsche S, Lupp A, Mackay F, Schulz S, Stumm R
PloS one (2012) 78: e42814. 132 002 IHC
Protein phosphatase 1 regulates the histone code for long-term memory.
Koshibu K, Gräff J, Beullens M, Heitz FD, Berchtold D, Russig H, Farinelli M, Bollen M, Mansuy IM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2009) 2941: 13079-89. 132 002 IHC
PBX transcription factors drive pulmonary vascular adaptation to birth.
McCulley DJ, Wienhold MD, Hines EA, Hacker TA, Rogers A, Pewowaruk RJ, Zewdu R, Chesler NC, Selleri L, Sun X
The Journal of clinical investigation (2018) 1282: 655-667. 132 002 IHC, IHC-P; tested species: mouse
Postsynaptic gephyrin clustering controls the development of adult-born granule cells in the olfactory bulb.
Deprez F, Pallotto M, Vogt F, Grabiec M, Virtanen MA, Tyagarajan SK, Panzanelli P, Fritschy JM
The Journal of comparative neurology (2015) 52313: 1998-2016. 132 002 EM
Granule cell ascending axon excitatory synapses onto Golgi cells implement a potent feedback circuit in the cerebellar granular layer.
Cesana E, Pietrajtis K, Bidoret C, Isope P, D'Angelo E, Dieudonné S, Forti L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3330: 12430-46. 132 002 EM; tested species: rat
Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites.
Trajkovic K, Dhaunchak AS, Goncalves JT, Wenzel D, Schneider A, Bunt G, Nave KA, Simons M
The Journal of cell biology (2006) 1726: 937-48. 132 002 EM
Cat. No.: 132 002
Amount: 200 µl
Price: $355.00
Adamtsl3 mediates DCC signaling to selectively promote GABAergic synapse function.
Cramer TML, Pinan-Lucarre B, Cavaccini A, Damilou A, Tsai YC, Bhat MA, Panzanelli P, Rama N, Mehlen P, Benke D, Karayannis T, et al.
Cell reports (2023) 428: 112947. 132 002 WB; tested species: mouse
Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants.
Zlatic SA, Werner E, Surapaneni V, Lee CE, Gokhale A, Singleton K, Duong D, Crocker A, Gentile K, Middleton F, Dalloul JM, et al.
Human molecular genetics (2023) : . 132 002 WB, IP; tested species: mouse
Pharmacological perturbation of CXCL1 signaling alleviates neuropathogenesis in a model of HEVA71 infection.
Gunaseelan S, Ariffin MZ, Khanna S, Ooi MH, Perera D, Chu JJH, Chua JJE
Nature communications (2022) 131: 890. 132 002 WB; tested species: rat
FEZ1 forms complexes with CRMP1 and DCC to regulate axon and dendrite development.
Chua JY, Ng SJ, Yagensky O, Wanker EE, Chua JJE
eNeuro (2021) : . 132 002 WB; tested species: rat
Phosphorylation on Ser 359 of the α2 subunit in GABA type A receptors down-regulates their density at inhibitory synapses.
Nakamura Y, Morrow DH, Nathanson AJ, Henley JM, Wilkinson KA, Moss SJ
The Journal of biological chemistry (2020) : . 132 002 WB; tested species: rat
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 132 002 WB; tested species: human
Adaptor protein-3 complex is required for Vangl2 trafficking and planar cell polarity of the inner ear.
Tower-Gilchrist C, Zlatic SA, Yu D, Chang Q, Wu H, Lin X, Faundez V, Chen P
Molecular biology of the cell (2019) : mbcE16080592. 132 002 WB; tested species: mouse
Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicity.
Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ
eLife (2019) 8: . 132 002 WB; tested species: mouse
The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting.
Akula AK, Zhang X, Viotti JS, Nestvogel D, Rhee JS, Ebrecht R, Reim K, Wouters F, Liepold T, Jahn O, Bogeski I, et al.
Frontiers in molecular neuroscience (2019) 12: 249. 132 002 WB, ICC; tested species: mouse
Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged γ-Aminobutyric Acid Receptor, Type A (GABAA), α2 Subunit Knock-in Mouse.
Nakamura Y, Morrow DH, Modgil A, Huyghe D, Deeb TZ, Lumb MJ, Davies PA, Moss SJ
The Journal of biological chemistry (2016) 29123: 12394-407. 132 002 WB
Mover is a homomeric phospho-protein present on synaptic vesicles.
Ahmed S, Wittenmayer N, Kremer T, Hoeber J, Kiran Akula A, Urlaub H, Islinger M, Kirsch J, Dean C, Dresbach T
PloS one (2013) 85: e63474. 132 002 WB
Neuronal palmitoyl acyl transferases exhibit distinct substrate specificity.
Huang K, Sanders S, Singaraja R, Orban P, Cijsouw T, Arstikaitis P, Yanai A, Hayden MR, El-Husseini A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2009) 238: 2605-15. 132 002 WB, ICC
SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery.
Zhu GD, Salazar G, Zlatic SA, Fiza B, Doucette MM, Heilman CJ, Levey AI, Faundez V, L'hernault SW
Molecular biology of the cell (2009) 204: 1223-40. 132 002 WB
Systemic proteome phenotypes reveal defective metabolic flexibility in Mecp2 mutants.
Zlatic SA, Werner E, Surapaneni V, Lee CE, Gokhale A, Singleton K, Duong D, Crocker A, Gentile K, Middleton F, Dalloul JM, et al.
Human molecular genetics (2023) : . 132 002 WB, IP; tested species: mouse
CLASP2 Links Reelin to the Cytoskeleton during Neocortical Development.
Dillon GM, Tyler WA, Omuro KC, Kambouris J, Tyminski C, Henry S, Haydar TF, Beffert U, Ho A
Neuron (2017) 936: 1344-1358.e5. 132 002 IP
Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity.
Rotmann A, Strand D, Martiné U, Closs EI
The Journal of biological chemistry (2004) 27952: 54185-92. 132 002 IP
The Calmodulin Binding Region of the Synaptic Vesicle Protein Mover Is Required for Homomeric Interaction and Presynaptic Targeting.
Akula AK, Zhang X, Viotti JS, Nestvogel D, Rhee JS, Ebrecht R, Reim K, Wouters F, Liepold T, Jahn O, Bogeski I, et al.
Frontiers in molecular neuroscience (2019) 12: 249. 132 002 WB, ICC; tested species: mouse
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 132 002 ICC
Neuronal palmitoyl acyl transferases exhibit distinct substrate specificity.
Huang K, Sanders S, Singaraja R, Orban P, Cijsouw T, Arstikaitis P, Yanai A, Hayden MR, El-Husseini A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2009) 238: 2605-15. 132 002 WB, ICC
Control of the postsynaptic membrane viscosity.
Renner M, Choquet D, Triller A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2009) 299: 2926-37. 132 002 ICC; tested species: rat
Activity-dependent ubiquitination of GABA(A) receptors regulates their accumulation at synaptic sites.
Saliba RS, Michels G, Jacob TC, Pangalos MN, Moss SJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2007) 2748: 13341-51. 132 002 ICC
Gephyrin regulates the cell surface dynamics of synaptic GABAA receptors.
Jacob TC, Bogdanov YD, Magnus C, Saliba RS, Kittler JT, Haydon PG, Moss SJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2545: 10469-78. 132 002 ICC
Presence of ethanol-sensitive and ethanol-insensitive glycine receptors in the ventral tegmental area and prefrontal cortex in mice.
Araya A, Gallegos S, Viveros R, San Martin L, Muñoz B, Harvey RJ, Zeilhofer HU, Aguayo LG
British journal of pharmacology (2021) 17823: 4691-4707. 132 002 IHC; tested species: mouse
Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content.
Hartwig C, Méndez GM, Bhattacharjee S, Vrailas-Mortimer AD, Zlatic SA, Freeman AAH, Gokhale A, Concilli M, Werner E, Sapp Savas C, Rudin-Rush S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) 412: 215-233. 132 002 IHC; tested species: drosophila
Temporal Dynamics and Neuronal Specificity of Grin3a Expression in the Mouse Forebrain.
Murillo A, Navarro AI, Puelles E, Zhang Y, Petros TJ, Pérez-Otaño I
Cerebral cortex (New York, N.Y. : 1991) (2020) : . 132 002 IHC; tested species: mouse
Semaphorin 2b Regulates Sleep-Circuit Formation in the Drosophila Central Brain.
Xie X, Tabuchi M, Corver A, Duan G, Wu MN, Kolodkin AL
Neuron (2019) 1042: 322-337.e14. 132 002 IHC
PBX transcription factors drive pulmonary vascular adaptation to birth.
McCulley DJ, Wienhold MD, Hines EA, Hacker TA, Rogers A, Pewowaruk RJ, Zewdu R, Chesler NC, Selleri L, Sun X
The Journal of clinical investigation (2018) 1282: 655-667. 132 002 IHC, IHC-P; tested species: mouse
Widespread transduction of astrocytes and neurons in the mouse central nervous system after systemic delivery of a self-complementary AAV-PHP.B vector.
Rincon MY, de Vin F, Duqué SI, Fripont S, Castaldo SA, Bouhuijzen-Wenger J, Holt MG
Gene therapy (2018) : . 132 002 IHC; tested species: mouse
An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody.
Batiuk MY, de Vin F, Duqué SI, Li C, Saito T, Saido T, Fiers M, Belgard TG, Holt MG
The Journal of biological chemistry (2017) 29221: 8874-8891. 132 002 IHC; tested species: mouse
Morphological study of a connexin 43-GFP reporter mouse highlights glial heterogeneity, amacrine cells, and olfactory ensheathing cells.
Theofilas P, Steinhäuser C, Theis M, Derouiche A
Journal of neuroscience research (2017) 9511: 2182-2194. 132 002 IHC; tested species: mouse
CXC chemokine receptor 7 (CXCR7) regulates CXCR4 protein expression and capillary tuft development in mouse kidney.
Haege S, Einer C, Thiele S, Mueller W, Nietzsche S, Lupp A, Mackay F, Schulz S, Stumm R
PloS one (2012) 78: e42814. 132 002 IHC
Protein phosphatase 1 regulates the histone code for long-term memory.
Koshibu K, Gräff J, Beullens M, Heitz FD, Berchtold D, Russig H, Farinelli M, Bollen M, Mansuy IM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2009) 2941: 13079-89. 132 002 IHC
PBX transcription factors drive pulmonary vascular adaptation to birth.
McCulley DJ, Wienhold MD, Hines EA, Hacker TA, Rogers A, Pewowaruk RJ, Zewdu R, Chesler NC, Selleri L, Sun X
The Journal of clinical investigation (2018) 1282: 655-667. 132 002 IHC, IHC-P; tested species: mouse
Postsynaptic gephyrin clustering controls the development of adult-born granule cells in the olfactory bulb.
Deprez F, Pallotto M, Vogt F, Grabiec M, Virtanen MA, Tyagarajan SK, Panzanelli P, Fritschy JM
The Journal of comparative neurology (2015) 52313: 1998-2016. 132 002 EM
Granule cell ascending axon excitatory synapses onto Golgi cells implement a potent feedback circuit in the cerebellar granular layer.
Cesana E, Pietrajtis K, Bidoret C, Isope P, D'Angelo E, Dieudonné S, Forti L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3330: 12430-46. 132 002 EM; tested species: rat
Neuron to glia signaling triggers myelin membrane exocytosis from endosomal storage sites.
Trajkovic K, Dhaunchak AS, Goncalves JT, Wenzel D, Schneider A, Bunt G, Nave KA, Simons M
The Journal of cell biology (2006) 1726: 937-48. 132 002 EM
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.