Synaptic Systems

Cat. No.: 219 017

Amount: 100 µg

Price: $420.00

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Cat. No. 219 017	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 200 up to 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): not tested yet EM: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Clone	481
Subtype	IgG
Immunogen	Recombinant protein corresponding to AA 1 to 1115 from mouse NCAM180 (UniProt Id: P13595)
Reactivity	Reacts with: rat (P13596), mouse (P13595). Other species not tested yet.
Specificity	Specific for NCAM180. Does not detect NCAM120 and NCAM140.
Remarks	This antibody lacks the Fc region and has to be used like a Fab fragment. Secondary reagents directed exclusively against the Fc region are not recommended. EM: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	Data Sheet 219_017 Safety Data Sheet (SDS)

References for NCAM180 - 219 017

Developmentally regulated masking of an intracellular epitope of the 180 kDa isoform of the neural cell adhesion molecule NCAM.
Kramer I, Hall H, Bleistein U, Schachner M
Journal of neuroscience research (1997) 492: 161-75. 219 017 WB, ICC, IHC

Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development.
Pollerberg EG, Sadoul R, Goridis C, Schachner M
The Journal of cell biology (1985) 1015 Pt 1: 1921-9. 219 017 WB, ICC, IHC

A multimodal 3D neuro-microphysiological system with neurite-trapping microelectrodes.
Molina-Martínez B, Jentsch LV, Ersoy F, van der Moolen M, Donato S, Ness TV, Heutink P, Jones PD, Cesare P
Biofabrication (2022) 142: . 219 017 ICC; tested species: human

Immunoelectron microscopic localization of the neural recognition molecules L1, NCAM, and its isoform NCAM180, the NCAM-associated polysialic acid, beta1 integrin and the extracellular matrix molecule tenascin-R in synapses of the adult rat hippocampus.
Schuster T, Krug M, Stalder M, Hackel N, Gerardy-Schahn R, Schachner M
Journal of neurobiology (2001) 492: 142-58. 219 017 IHC, EM

Cat. No.: 219 017

Amount: 100 µg

Price: $420.00

WB
(1 img)

ICC
(1 img)

IHC
(5 img)

ATLAS
(1 img)

Background

The neural cell adhesion molecule (NCAM) is a cell surface glycoprotein that occurs in several isoforms. It belongs to the immunglobulin superfamily of adhesion molecules and contains several immunglobulin-like domains. The largest, NCAM 180, may play an important role in the development and stabilization of cell contacts. It has been shown to accumulate at those sites of contact and at postsynaptic densities.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Becker CG et al., Exp. Neurol. (2001)
2 ) Delius JA et al., J. Neurosci. Res. (1997)

Protocols

ICC

Fixed Cells

IHC

NCAM180 (CD56) antibody - 219 017

References for NCAM180 - 219 017