Synaptic Systems - Munc13-3

Cat. No.: 126 303

Amount: 50 µg

Price: $380.00

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Cat. No. 126 303	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 22 to 332 from rat Munc13-3 (UniProt Id: Q62770)
Reactivity	Reacts with: rat (Q62770), mouse (Q8K0T7). Other species not tested yet.
Specificity	K.O. validated
Data sheet	Data Sheet 126_303 Safety Data Sheet (SDS)

References for Munc13-3 - 126 303

Chronic pharmacologic manipulation of dopamine transmission ameliorates metabolic disturbance in trappc9-linked brain developmental syndrome.
Li Y, Muhammad U, Sapp E, Ke Y, Wang Z, Boudi A, DiFiglia M, Li X
JCI insight (2024) : . 126 303 WB; tested species: mouse

Chronic social isolation stress during peri-adolescence alters presynaptic dopamine terminal dynamics via augmentation in accumbal dopamine availability.
Karkhanis AN, Leach AC, Yorgason JT, Uneri A, Barth S, Niere F, Alexander NJ, Weiner JL, McCool BA, Raab-Graham KF, Ferris MJ, et al.
ACS chemical neuroscience (2018) : . 126 303 WB; tested species: rat

Cat. No.: 126 303

Amount: 50 µg

Price: $380.00

WB
(1 img)

Background

Munc13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc13-2 is mainly expressed in cortex and hippocampus whereas Munc13-3 exhibits highest expression levels in cerebellum and pons.

Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described. Munc13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.

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6 ) Betz A et al., J. Biol. Chem. (1997)
7 ) Südhof TC et al., Nature (1995)
8 ) Brose N et al., J. Biol. Chem. (1995)
9 ) Goldenberg NM et al., Am. J. Physiol., Cell Physiol. (2009)
10 ) Zhu Y et al., J. Physiol. (Lond.) (2008)

Protocols

Munc13-3 antibody - 126 303 K.O.

References for Munc13-3 - 126 303