Synaptic Systems - Munc13-2

Cat. No.: 126 203

Amount: 50 µg

Price: $380.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

Cat. No. 126 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: external data (see remarks) gallery IHC-P (FFPE): not tested yet EM: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Immunogen	Recombinant protein corresponding to AA 151 to 317 from rat Munc13-2 isoform 2 (UniProt Id: Q62769-2)
Reactivity	Reacts with: rat (Q62769), mouse (Q9Z1N9). Other species not tested yet.
Specificity	Specific for munc 13-2 brain specific isoform. K.O. validated
Remarks	IHC: This antibody has been successfully applied for this method by our customers using mild fixation (1% PFA at pH 6) according to Lorincz and Nusser 2010 (see gallery). It has not been validated using our standard protocol. EM: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	Data Sheet 126_203 Safety Data Sheet (SDS)

References for Munc13-2 - 126 203

Dynamic recruitment of Munc13 primes docked secretory granules for exocytosis.
Echeverry S, Saras J, Lund PE, Dunevall J, Gandasi NR, Barg S
Cell reports (2025) 4410: 116301. 126 203 WB, ICC; tested species: rat

Loss of synaptic Munc13-1 underlies neurotransmission abnormalities in spinal muscular atrophy.
Moradi M, Deng C, Sendtner M
Cellular and molecular life sciences : CMLS (2025) 821: 325. 126 203 WB, ICC, IHC; tested species: mouse

Differential Expression of Munc13-2 Produces Unique Synaptic Phenotypes in the Basolateral Amygdala of C57BL/6J and DBA/2J Mice.
Gioia DA, Alexander NJ, McCool BA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3643: 10964-10977. 126 203 WB

Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 126 203 IHC; KD verified; tested species: mouse,rat

RIM-BP2 primes synaptic vesicles via recruitment of Munc13-1 at hippocampal mossy fiber synapses.
Brockmann MM, Maglione M, Willmes CG, Stumpf A, Bouazza BA, Velasquez LM, Grauel MK, Beed P, Lehmann M, Gimber N, Schmoranzer J, et al.
eLife (2019) 8: . 126 203 IHC; tested species: mouse

Light-microscopy-based connectomic reconstruction of mammalian brain tissue.
Tavakoli MR, Lyudchik J, Januszewski M, Vistunou V, Agudelo Dueñas N, Vorlaufer J, Sommer C, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, et al.
Nature (2025) 6428067: 398-410. 126 203 ExM; tested species: mouse

Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons.
Kim O, Okamoto Y, Kaufmann WA, Brose N, Shigemoto R, Jonas P
PLoS biology (2024) 2211: e3002879. 126 203 EM; tested species: mouse,rat

GRASPing experience-dependent protein expression signatures enriched for hippocampal engram cell synapses.
Moharana B, Nemat P, Pullen RM, Gradl A, Schipper M, Savage JE, Klaassen RV, van der Loo RJ, Chadick CH, Koopmans F, Gouwenberg Y, et al.
Science advances (2026) 1220: eadv3557. 126 203 FACS; tested species: mouse

Cat. No.: 126 203

Amount: 50 µg

Price: $380.00

WB
(1 img)

IHC
(3 img)

Background

Munc13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc13-2 is mainly expressed in cortex and hippocampus whereas Munc13-3 exhibits highest expression levels in cerebellum and pons.

Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described. Munc13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.

1 ) Sheu L et al., J. Biol. Chem. (2003)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Ohtsuka T et al., J. Cell Biol. (2002)
4 ) Ashery U et al., EMBO J. (2000)
5 ) Brose N et al., Curr. Opin. Neurobiol. (2000)
6 ) Betz A et al., J. Biol. Chem. (1997)
7 ) Südhof TC et al., Nature (1995)
8 ) Brose N et al., J. Biol. Chem. (1995)
9 ) Goldenberg NM et al., Am. J. Physiol., Cell Physiol. (2009)
10 ) Zhu Y et al., J. Physiol. (Lond.) (2008)

Protocols

IHC

see remarks

Other products for this target

Munc13-2 antibody - 126 203 K.O.

References for Munc13-2 - 126 203