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Complexin2 antibody SNARE binding domain - 122 103

Complexins are nerve-terminal syntaxin binding proteins
Rabbit polyclonal purified antibody
Cat. No.: 122 103
Amount: 50 µg
Price: $380.00
Cat. No. 122 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not recommended

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunogen Synthetic peptide within the SNARE binding domain of mouse Complexin2 (UniProt Id: P84086)
Reactivity Reacts with: mouse (P84086), rat (P84087).
Other species not tested yet.
Predicted to cross-react with human (Q6PUV4) due to high sequence homology.
Specificity Antibody has a strong preference for Complexin2 and shows only minor cross-reactivity with Complexin1.
Data sheet 122_103.pdf
Cat. No.: 122 103
Amount: 50 µg
Price: $380.00

Complexin2 (red) and Synaptoporin (green) staining in a mouse hippocampus section