Tailor-made Antibodies
and Tools for Life Science
Home|||||||Technical Support|

Complexin 1/2 antibody - 122 004

Complexins are nerve-terminal syntaxin binding proteins
Guinea pig polyclonal antiserum
Cat. No.: 122 004
Amount: 100 µl
Price: $350.00
Cat. No. 122 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 122 to 134 from human Complexin2 (UniProt Id: Q6PUV4)
Reactivity Reacts with: human (O14810, Q6PUV4), rat (P63041, P84087), mouse (P63040, P84086).
Other species not tested yet.
Specificity Recognizes complexin 1 and 2. K.O. PubMed: 34360929
Matching control protein/peptide 122-0P
Data sheet 122_004.pdf

References for Complexin 1/2 - 122 004

Cell Types and Synapses Expressing the SNARE Complex Regulating Proteins Complexin 1 and Complexin 2 in Mammalian Retina.
Lux UT, Ehrenberg J, Joachimsthaler A, Atorf J, Pircher B, Reim K, Kremers J, Gießl A, Brandstätter JH
International journal of molecular sciences (2021) 2215: . 122 004 IHC; KO verified; tested species: mouse
Cat. No.: 122 004
Amount: 100 µl
Price: $350.00
Cell Types and Synapses Expressing the SNARE Complex Regulating Proteins Complexin 1 and Complexin 2 in Mammalian Retina.
Lux UT, Ehrenberg J, Joachimsthaler A, Atorf J, Pircher B, Reim K, Kremers J, Gießl A, Brandstätter JH
International journal of molecular sciences (2021) 2215: . 122 004 IHC; KO verified; tested species: mouse
Background

Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2, also referred to as synaphin 1, is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 (synaphin 2) and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.