Munc18-3 antibody - 116 203

Munc18 proteins are peripheral membrane protein involved in vesicle docking and fusion

Rabbit polyclonal purified antibody

Cat. No.: 116 203

Amount: 50 µg

Price: $460.00

Cat. No. 116 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 575 to 593 from rat Munc18-3 (UniProt Id: Q99PV2)
Reactivity	Reacts with: rat (Q99PV2). No signal: zebrafish. Other species not tested yet.
Specificity	Specific for Munc 18-3, no cross-reactivity to Munc 18-1 and Munc 18-2.
Matching control protein/peptide	116-2P
Data sheet	Datasheet 116_203

Cat. No.: 116 203

Amount: 50 µg

Price: $460.00

Background

Munc 18 is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin 1. It is highly homologous to the C. elegans unc-18 gene product, and weakly related to the yeast sec1, sly1, and slp1 genes.
There are three munc 18 isoforms in mammals. Munc 18-1 or 18a, also referred to as rb-sec1, n-sec1, stxbp1 and p67, is primarily expressed in neurons. Munc 18-2 or 18b, also referred to as stxbp2, and Munc 18-3 or 18c are expressed ubiquitously.

1 ) Tellam JT et al., J. Biol. Chem. (1995)
2 ) Hata Y et al., J. Biol. Chem. (1995)
3 ) Pevsner J et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
4 ) Garcia EP et al., Proc. Natl. Acad. Sci. U.S.A. (1994)
5 ) Hata Y et al., Nature (1993)
6 ) Fukuda M et al., J. Biol. Chem. (2005)
7 ) Martin-Verdeaux S et al., J. Cell. Sci. (2003)
8 ) Riento K et al., J. Biol. Chem. (2000)

Protocols

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