|Cat. No. 112-2P||
100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
|Immunogen||Synthetic peptide corresponding to AA 283 to 298 from mouse βSNAP (UniProt Id: P28663)|
|Recommended dilution||Optimal concentrations should be determined by the end-user.|
|Matching antibodies||112 203|
This control peptide consists of the synthetic peptide (IKKSIQGDGEGDGDLK) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
The proteins α/β-SNAP are two related soluble and highly conserved proteins that bind to the fusion complex (SNARE complex), thus allowing the N-ethylmaleimide sensitive fusion protein NSF to bind to the complex. γ-SNAP binds directly to NSF and Gaf-1/Rip11, a protein of the Rab11 interacting family. In contrast to α/β-SNAP it does not interact directly with SNARE proteins and is not required for ER-Golgi transport. SNAP-proteins are abundantly expressed in all tissues. They are partially soluble, partially membrane-bound.