Synaptic Systems

Cat. No.: 111 302

Amount: 200 µl

Price: $360.00

Cat. No. 111 302	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: external data (see remarks) IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 257 from rat SNAP29 (UniProt Id: Q9Z2P6)
Reactivity	Reacts with: human (O95721), rat (Q9Z2P6), mouse (Q9ERB0), hamster. Other species not tested yet.
Specificity	K.O. validated
Remarks	ICC: This antibody has been successfully applied and published for this method by customers (see application-specific references). It has not been validated using our standard protocols. The affinity purified antibody (cat. no. 111 303) is recommended.
Data sheet	Datasheet 111_302

References for SNAP29 - 111 302

SNARE-binding protein synaptosomal-associated protein of 29 kDa (SNAP29) regulates the intracellular sequestration of glucose transporter 4 (GLUT4) vesicles in adipocytes.
Matsui K, Emoto M, Fukuda N, Nomiyama R, Yamada K, Tanizawa Y
Journal of diabetes investigation (2022) : . 111 302 WB, IP, ICC; tested species: mouse

Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons.
Arioka Y, Shishido E, Kushima I, Suzuki T, Saito R, Aiba A, Mori D, Ozaki N
EBioMedicine (2020) 63: 103138. 111 302 WB; tested species: mouse

Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth.
Wojnacki J, Nola S, Bun P, Cholley B, Filippini F, Pressé MT, Lipecka J, Man Lam S, N'guyen J, Simon A, Ouslimani A, et al.
Cell reports (2020) 3312: 108536. 111 302 WB, ICC; tested species: rat

Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17.
Matsui T, Jiang P, Nakano S, Sakamaki Y, Yamamoto H, Mizushima N
The Journal of cell biology (2018) 2178: 2633-2645. 111 302 WB; KD verified; tested species: human

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 302 WB; tested species: rat

Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 111 302 WB, IP

Phosphorylation of SNAP-23 in activated human platelets.
Polgár J, Lane WS, Chung SH, Houng AK, Reed GL
The Journal of biological chemistry (2003) 27845: 44369-76. 111 302 WB; tested species: human

Cat. No.: 111 302

Amount: 200 µl

Price: $360.00

Background

SNAP29, also known as GS32, is an ubiquitously distributed relative of SNAP25 and SNAP23 that is ubiquitously distributed among intracellular membranes and that is also found in the cytosol of mammalian cells. As a Q-SNARE it forms SNARE complexes in vitro but its precise role in intracellular membrane traffic is not known.

1 ) Antonin W et al., EMBO J. (2000)
2 ) Pabst S et al., J. Biol. Chem. (2000)
3 ) Wong SH et al., Mol. Biol. Cell (1999)
4 ) Jahn R et al., Annu. Rev. Biochem. (1999)
5 ) Steegmaier M et al., J. Biol. Chem. (1998)

Protocols

ICC

see remarks

Standard Condition

Other products for this target

SNAP29 antibody - 111 302 K.O.

References for SNAP29 - 111 302