|Cat. No. 111 108||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 10000 (AP staining) gallery
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1)|
Epitop: AA 1 to 20 from rat SNAP25B (UniProt Id: P60881-1)
Reacts with: human (P60880), rat (P60881-1), mouse (P60879), mammals, bovine.
No signal: zebrafish.
Other species not tested yet.
|Specificity||Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products.|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 71.2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
SNAP 25 (synaptosome-associated protein of 25 kDa) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP 25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP 25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin 1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP 25A and 25B.