Syntaxin16 antibody - 110 163

Syntaxin16 is a SNARE protein involved in trans-Golgi network trafficking

Rabbit polyclonal purified antibody

Cat. No.: 110 163

Amount: 50 µg

Price: $460.00

Cat. No. 110 163	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet ELISA: Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Recombinant protein corresponding to AA 1 to 302 from rat Syntaxin16 (UniProt Id: A0A0G2K528)
Reactivity	Reacts with: human (O14662), rat (A0A0G2K528), mouse (Q8BVI5), hamster. Other species not tested yet.
Specificity	Recognizes all four isoforms syntaxin 16a, b, c, d.
Matching control protein/peptide	110-16P
Data sheet	Datasheet 110_163

References for Syntaxin16 - 110 163

Sugar transporter Slc37a2 regulates bone metabolism in mice via a tubular lysosomal network in osteoclasts.
Ng PY, Ribet ABP, Guo Q, Mullin BH, Tan JWY, Landao-Bassonga E, Stephens S, Chen K, Yuan J, Abudulai L, Bollen M, et al.
Nature communications (2023) 141: 906. 110 163 WB; tested species: mouse

Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons.
van Bommel DM, Toonen RF, Verhage M
Scientific reports (2023) 131: 2871. 110 163 ICC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 110 163 DNA-PAINT; tested species: rat

Cat. No.: 110 163

Amount: 50 µg

Price: $460.00

ICC

Background

Syntaxin 16, a member of the SNARE family of proteins, localizes to the Golgi stack. It has been shown to be involved in trans-Golgi network trafficking and to interact with VAMP 3, VAMP 4 and VAMP 8.
Four splice variants (syntaxin 16a, b, c, d) have been described, which may have different roles in intracellular trafficking. The splice variant c is the shortest and localizes to the cytoplasm.

1 ) Xu H et al., J. Cell. Sci. (2002)
2 ) Simonsen A et al., Eur. J. Cell Biol. (1998)
3 ) Tang BL et al., Biochem. Biophys. Res. Commun. (1998)

Protocols

ICC

Fixed Cells

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