Synaptic Systems - Syntaxin2

Cat. No.: 110 123

Amount: 50 µg

Price: $460.00

Cat. No. 110 123	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 265 from rat Syntaxin2 (UniProt Id: P50279)
Reactivity	Reacts with: human (P32856), rat (P50279), mouse (Q00262), hamster. Other species not tested yet.
Specificity	K.D. validated PubMed: 36316316
Matching control protein/peptide	110-2P
Data sheet	Datasheet 110_123

References for Syntaxin2 - 110 123

The stability of the primed pool of synaptic vesicles and the clamping of spontaneous neurotransmitter release rely on the integrity of the C-terminal half of the SNARE domain of syntaxin-1A.
Salazar Lázaro A, Trimbuch T, Vardar G, Rosenmund C
eLife (2024) 12: . 110 123 WB, ICC; tested species: mouse

Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 110 123 WB, IP; KD verified; tested species: human,mouse

SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.
Liang T, Qin T, Kang F, Kang Y, Xie L, Zhu D, Dolai S, Greitzer-Antes D, Baker RK, Feng D, Tuduri E, et al.
JCI insight (2020) 53: . 110 123 WB; tested species: human,mouse

Melanophilin Accelerates Insulin Granule Fusion Without Predocking to the Plasma Membrane.
Wang H, Mizuno K, Takahashi N, Kobayashi E, Shirakawa J, Terauchi Y, Kasai H, Okunishi K, Izumi T
Diabetes (2020) : . 110 123 WB; tested species: mouse

Invasion by activated macrophages requires delivery of nascent MT1-MMP through late endosomes/lysosomes to the cell surface.
Röhl J, West ZE, Rudolph M, Zaharia A, Van Lonkhuyzen D, Hickey DK, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2019) : . 110 123 WB; tested species: mouse

Complexin 2 modulates vesicle-associated membrane protein (VAMP) 2-regulated zymogen granule exocytosis in pancreatic acini.
Falkowski MA, Thomas DD, Groblewski GE
The Journal of biological chemistry (2010) 28546: 35558-66. 110 123 WB

Syntaxin-2 balances phagocytic uptake and phagolysosomal clearance in macrophages.
Samanta S, Nandi A, Datta R, Dolai S
Journal of cell science (2025) : . 110 123 ICC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 110 123 DNA-PAINT; tested species: rat

Cat. No.: 110 123

Amount: 50 µg

Price: $460.00

Syntaxin-2 balances phagocytic uptake and phagolysosomal clearance in macrophages.
Samanta S, Nandi A, Datta R, Dolai S
Journal of cell science (2025) : . 110 123 ICC; tested species: mouse

ICC

Background

Syntaxin 2, also referred to as Epimorphin, a member of the SNARE family of proteins, is related to syntaxin 1. Like syntaxin 4 it is predominantly localized to the plasma membrane of a wide variety of cells.
Similar to syntaxins 1, 3 and 4, it appears to be involved in the fusion of transport vesicles with the plasma membrane.

1 ) Jahn R et al., Annu. Rev. Biochem. (1999)
2 ) Bennett MK et al., Cell (1993)
3 ) Hirai Y et al., Cell (1992)

Protocols

ICC

Fixed Cells

Standard Condition

Other products for this target

Syntaxin2 antibody - 110 123 K.D.

References for Syntaxin2 - 110 123