Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 105 3FB

Synaptotagmin1 antibody luminal domain - 105 3FB

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal Fab1 fragment
Cat. No.: 105 3FB
Amount: 100 µg
Price: $605.00
Cat. No. 105 3FB 100 µg purified monoclonal Fab1 fragment, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: yes (see remarks)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
Clone 604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096), zebrafish.
Other species not tested yet.
Remarks

WB: The full IgG (cat. no. 105 311) is recommended.
ICC: A Fab-specific secondary reagent is recommended for detection.
Data sheet 105_3fb.pdf
Cat. No.: 105 3FB
Amount: 100 µg
Price: $605.00
ICC
ICC
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
Other products for this target