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Synaptotagmin1 antibody luminal domain - 105 211 K.O.

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal purified IgG
Cat. No.: 105 211
Amount: 100 µg
Price: $415.00
Cat. No. 105 211 100 µg purified IgG, lyophilized. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining ) (see remarks) gallery  
IP: yes
ICC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC: 1 : 500 (see remarks)
IHC-P: not tested yet
Clone 604.1
Subtype IgG3 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707), mouse (P46096).
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated
Remarks

WB: Only detects rat Synaptotagmin1 in Western blots.
ICC: Antibody is suitable for the labeling of recycling vesicles of living rat and mouse neurons. It detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1.
IHC: Antibody detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1.

Data sheet 105_211.pdf
Cat. No.: 105 211
Amount: 100 µg
Price: $415.00
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.