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Synaptotagmin5/9 antibody - 105 053 K.O.

Synaptotagmin5/9 is predominantly expressed in brain
Rabbit polyclonal purified antibody
Cat. No.: 105 053
Amount: 50 µg
Price: $375.00
Cat. No. 105 053 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 50 to 108 from mouse Synaptotagmin5 (UniProt Id: Q9R0N5) (UniProt Id: Q9R0N5)
Reactivity Reacts with: rat (P47861), mouse (Q9R0N5).
Other species not tested yet.
Specificity Specific for synaptotagmin 5 (UniProt Id: Q9R0N5) sometimes referred to as synaptotagmin 9. Does not detect (UniProt Id: Q9R0N9); K.O. validated PubMed: 37432648
Data sheet 105_053.pdf

References for Synaptotagmin5/9 - 105 053

Palmitoylation couples insulin hypersecretion with β cell failure in diabetes.
Dong G, Adak S, Spyropoulos G, Zhang Q, Feng C, Yin L, Speck SL, Shyr Z, Morikawa S, Kitamura RA, Kathayat RS, et al.
Cell metabolism (2023) : . 105 053 WB; tested species: rat
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 105 053 WB, ICC; KO verified; tested species: mouse
Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.
Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 439: 1475-1491. 105 053 WB, ICC; KO verified; tested species: mouse
Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 105 053 WB; tested species: rat
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 105 053 WB
Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen.
Dean C, Dunning FM, Liu H, Bomba-Warczak E, Martens H, Bharat V, Ahmed S, Chapman ER
Molecular biology of the cell (2012) 239: 1715-27. 105 053 WB, ICC
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 105 053 WB, ICC; KO verified; tested species: mouse
Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.
Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 439: 1475-1491. 105 053 WB, ICC; KO verified; tested species: mouse
Sex-specific regulation of follicle-stimulating hormone secretion by synaptotagmin 9.
Roper LK, Briguglio JS, Evans CS, Jackson MB, Chapman ER
Nature communications (2015) 6: 8645. 105 053 ICC
Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen.
Dean C, Dunning FM, Liu H, Bomba-Warczak E, Martens H, Bharat V, Ahmed S, Chapman ER
Molecular biology of the cell (2012) 239: 1715-27. 105 053 WB, ICC
GABAergic-like dopamine synapses in the brain.
Kim HJ, Hwang B, Reva M, Lee J, Lee BE, Lee Y, Cho EJ, Jeong M, Lee SE, Myung K, Baik JH, et al.
Cell reports (2023) 4210: 113239. 105 053 IHC; tested species: mouse
Cat. No.: 105 053
Amount: 50 µg
Price: $375.00
Palmitoylation couples insulin hypersecretion with β cell failure in diabetes.
Dong G, Adak S, Spyropoulos G, Zhang Q, Feng C, Yin L, Speck SL, Shyr Z, Morikawa S, Kitamura RA, Kathayat RS, et al.
Cell metabolism (2023) : . 105 053 WB; tested species: rat
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 105 053 WB, ICC; KO verified; tested species: mouse
Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.
Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 439: 1475-1491. 105 053 WB, ICC; KO verified; tested species: mouse
Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 105 053 WB; tested species: rat
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 105 053 WB
Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen.
Dean C, Dunning FM, Liu H, Bomba-Warczak E, Martens H, Bharat V, Ahmed S, Chapman ER
Molecular biology of the cell (2012) 239: 1715-27. 105 053 WB, ICC
Genetic ablation of synaptotagmin-9 alters tomosyn-1 function to increase insulin secretion from pancreatic β-cells improving glucose clearance.
Rahman MM, Pathak A, Schueler KL, Alsharif H, Michl A, Alexander J, Kim JA, Bhatnagar S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2023) 378: e23075. 105 053 WB, ICC; KO verified; tested species: mouse
Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.
Seibert MJ, Evans CS, Stanley KS, Wu Z, Chapman ER
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 439: 1475-1491. 105 053 WB, ICC; KO verified; tested species: mouse
Sex-specific regulation of follicle-stimulating hormone secretion by synaptotagmin 9.
Roper LK, Briguglio JS, Evans CS, Jackson MB, Chapman ER
Nature communications (2015) 6: 8645. 105 053 ICC
Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen.
Dean C, Dunning FM, Liu H, Bomba-Warczak E, Martens H, Bharat V, Ahmed S, Chapman ER
Molecular biology of the cell (2012) 239: 1715-27. 105 053 WB, ICC
GABAergic-like dopamine synapses in the brain.
Kim HJ, Hwang B, Reva M, Lee J, Lee BE, Lee Y, Cho EJ, Jeong M, Lee SE, Myung K, Baik JH, et al.
Cell reports (2023) 4210: 113239. 105 053 IHC; tested species: mouse
Background
Up to now at least 17 synaptotagmins have been identified. Two different synaptotagmins were independently termed Synaptotagmin 5 by two research groups (see Li et al., 1995 and Craxton & Goeder, 1995). The groups refer to the respective other protein as Synaptotagmin 9. The synaptotagmin described by Craxton & Goeder is expressed in different tissues like kidney, heart, lung and adipose tissue but shows its highest expression level in brain, where it predominantly locates to an enriched synaptic vesicle fraction. Like other synaptotagmins it contains one transmembrane and two cytosolic C2 domains.