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Synaptotagmin 1/2 antibody cytoplasmic tail - 105 002

Synaptotagmin1/2 are Ca2+-sensors on synaptic vesicles that trigger neurotransmitter release
Rabbit polyclonal antiserum
Cat. No.: 105 002
Amount: 200 µl
Price: $350.00
Cat. No. 105 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: 1 : 400 gallery  
ELISA: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 120 to 131 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: human (P21579), rat (P21707), mouse (P46096), cow, chicken, goldfish, zebrafish.
Other species not tested yet.
[[105002-zebrafish-ihc.jpg]]
Specificity Some cross-reactivity to synaptotagmin 2.
Matching control protein/peptide 105-0P
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 105 011 as capture antibodies. The ELISA-protocol for membrane proteins is recommended.

Data sheet 105_002.pdf

References for Synaptotagmin 1/2 - 105 002

APC/CCdh1-Rock2 pathway controls dendritic integrity and memory.
Bobo-Jiménez V, Delgado-Esteban M, Angibaud J, Sánchez-Morán I, de la Fuente A, Yajeya J, Nägerl UV, Castillo J, Bolaños JP, Almeida A
Proceedings of the National Academy of Sciences of the United States of America (2017) 11417: 4513-4518. 105 002 WB
Membrane-tethered monomeric neurexin LNS-domain triggers synapse formation.
Gokce O, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3336: 14617-28. 105 002 WB; tested species: mouse
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 105 002 WB; tested species: mouse
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 105 002 WB; tested species: rat
Synaptotagmin I and II are present in distinct subsets of central synapses.
Fox MA, Sanes JR
The Journal of comparative neurology (2007) 5032: 280-96. 105 002 WB; tested species: zebrafish
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Volmer R, Monnet C, Gonzalez-Dunia D
PLoS pathogens (2006) 23: e19. 105 002 ICC, WB; tested species: rat
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 105 002 WB; tested species: mouse
Developmental control of synaptic receptivity.
Barker AJ, Koch SM, Reed J, Barres BA, Ullian EM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2833: 8150-60. 105 002 ICC; tested species: rat
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Volmer R, Monnet C, Gonzalez-Dunia D
PLoS pathogens (2006) 23: e19. 105 002 ICC, WB; tested species: rat
Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain.
Delignat-Lavaud B, Ducrot C, Kouwenhoven W, Feller N, Trudeau LÉ
Open biology (2022) 123: 210339. 105 002 IHC; tested species: mouse
Critical role of JSAP1 and JLP in axonal transport in the cerebellar Purkinje cells of mice.
Sato T, Ishikawa M, Yoshihara T, Nakazato R, Higashida H, Asano M, Yoshioka K
FEBS letters (2015) 58919 Pt B: 2805-11. 105 002 IHC
Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals.
Rodriguez-Gil DJ, Hu W, Greer CA
PloS one (2013) 82: e56561. 105 002 IHC; tested species: mouse
Cat. No.: 105 002
Amount: 200 µl
Price: $350.00
APC/CCdh1-Rock2 pathway controls dendritic integrity and memory.
Bobo-Jiménez V, Delgado-Esteban M, Angibaud J, Sánchez-Morán I, de la Fuente A, Yajeya J, Nägerl UV, Castillo J, Bolaños JP, Almeida A
Proceedings of the National Academy of Sciences of the United States of America (2017) 11417: 4513-4518. 105 002 WB
Membrane-tethered monomeric neurexin LNS-domain triggers synapse formation.
Gokce O, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3336: 14617-28. 105 002 WB; tested species: mouse
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 105 002 WB; tested species: mouse
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 105 002 WB; tested species: rat
Synaptotagmin I and II are present in distinct subsets of central synapses.
Fox MA, Sanes JR
The Journal of comparative neurology (2007) 5032: 280-96. 105 002 WB; tested species: zebrafish
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Volmer R, Monnet C, Gonzalez-Dunia D
PLoS pathogens (2006) 23: e19. 105 002 ICC, WB; tested species: rat
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 105 002 WB; tested species: mouse
Developmental control of synaptic receptivity.
Barker AJ, Koch SM, Reed J, Barres BA, Ullian EM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2833: 8150-60. 105 002 ICC; tested species: rat
Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Volmer R, Monnet C, Gonzalez-Dunia D
PLoS pathogens (2006) 23: e19. 105 002 ICC, WB; tested species: rat
Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain.
Delignat-Lavaud B, Ducrot C, Kouwenhoven W, Feller N, Trudeau LÉ
Open biology (2022) 123: 210339. 105 002 IHC; tested species: mouse
Critical role of JSAP1 and JLP in axonal transport in the cerebellar Purkinje cells of mice.
Sato T, Ishikawa M, Yoshihara T, Nakazato R, Higashida H, Asano M, Yoshioka K
FEBS letters (2015) 58919 Pt B: 2805-11. 105 002 IHC
Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals.
Rodriguez-Gil DJ, Hu W, Greer CA
PloS one (2013) 82: e56561. 105 002 IHC; tested species: mouse
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.