Synaptic Systems

Cat. No.: 237 002

Amount: 200 µl

Price: $360.00

Cat. No. 237 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 up to 1 : 2000 gallery IHC: not recommended IHC-P: 1 : 200 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 13 from human EEA1 (UniProt Id: Q15075)
Reactivity	Reacts with: human (Q15075), rat (A0A0G2K051), mouse (Q8BL66). No signal: zebrafish. Other species not tested yet.
Data sheet	237_002.pdf

References for EEA1 - 237 002

Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 237 002 WB

Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 237 002 WB

C9orf72 hexanucleotide repeat expansions impair microglial response in ALS.
Masrori P, Bijnens B, Fumagalli L, Davie K, Poovathingal SK, Meese T, Storm A, Hersmus N, Fazal R, van den Biggelaar D, Asselbergh B, et al.
Nature neuroscience (2025) : . 237 002 ICC; tested species: human

Coupling of microtubule motors with AP-3 generated organelles in axons by NEEP21 Family Member Calcyon.
Shi L, Hines T, Bergson C, Smith D
Molecular biology of the cell (2018) : mbcE18010007. 237 002 ICC; tested species: monkey

A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 237 002 ICC

Sorting in early endosomes reveals connections to docking- and fusion-associated factors.
Barysch SV, Aggarwal S, Jahn R, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2009) 10624: 9697-702. 237 002

SNARE function is not involved in early endosome docking.
Geumann U, Barysch SV, Hoopmann P, Jahn R, Rizzoli SO
Molecular biology of the cell (2008) 1912: 5327-37. 237 002

Cat. No.: 237 002

Amount: 200 µl

Price: $360.00

SNARE function is not involved in early endosome docking.
Geumann U, Barysch SV, Hoopmann P, Jahn R, Rizzoli SO
Molecular biology of the cell (2008) 1912: 5327-37. 237 002

ICC

IHC-P

Background

Extracellular compounds are internalized by endocytosis into so called endocytic vesicles. They fuse with early endosomes, from where the endocytosed material can be shuttled to a number of alternative destinations.
Early endosomal antigen 1 (EEA 1) is a peripheral membrane protein that locates to early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of Rab5.
Autoantibodies against EEA 1 have been shown to be associated with subacute cutaneous systemic lupus erythematosus.

1 ) Bergeland T et al., EMBO Rep. (2008)
2 ) Wilson JM et al., Mol. Biol. Cell (2000)
3 ) Simonsen A et al., J. Biol. Chem. (1999)
4 ) Callaghan J et al., Biochem. J. (1999)
5 ) Stenmark H et al., J. Biol. Chem. (1996)
6 ) Mu FT et al., J. Biol. Chem. (1995)

Protocols

ICC

IHC-P

Other products for this target

EEA1 antibody - 237 002

References for EEA1 - 237 002