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VGLUT1 antibody - 135 307 K.O.

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Goat polyclonal antiserum
Cat. No.: 135 307
Amount: 200 µl
Price: $330.00
Cat. No. 135 307 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 1000 up to 1 : 10000 gallery  
IHC: 1 : 300 gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 135-3P
Remarks

This antibody is highly recommended as a marker for glutamatergic nerve terminals.
WB:  VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
This antibody is highly recommended as marker for glutamatergic nerve terminals and gives excellent results in ICC.

Data sheet 135_307.pdf

References for VGLUT1 - 135 307

CDH2 mutation affecting N-cadherin function causes attention-deficit hyperactivity disorder in humans and mice.
Halperin D, Stavsky A, Kadir R, Drabkin M, Wormser O, Yogev Y, Dolgin V, Proskorovski-Ohayon R, Perez Y, Nudelman H, Stoler O, et al.
Nature communications (2021) 121: 6187. 135 307 ICC; tested species: mouse
Aberrant activity of mitochondrial NCLX is linked to impaired synaptic transmission and is associated with mental retardation.
Stavsky A, Stoler O, Kostic M, Katoshevsky T, Assali EA, Savic I, Amitai Y, Prokisch H, Leiz S, Daumer-Haas C, Fleidervish I, et al.
Communications biology (2021) 41: 666. 135 307 ICC; tested species: mouse
PSD-95 promotes the stabilization of young synaptic contacts.
Taft CE, Turrigiano GG
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691633: 20130134. 135 307 ICC; tested species: rat
Synaptic Targets of Glycinergic Neurons in Laminae I-III of the Spinal Dorsal Horn.
Miranda CO, Hegedüs K, Kis G, Antal M
International journal of molecular sciences (2023) 248: . 135 307 IHC; tested species: mouse
Visualizing Synaptic Multi-Protein Patterns of Neuronal Tissue With DNA-Assisted Single-Molecule Localization Microscopy.
Narayanasamy KK, Stojic A, Li Y, Sass S, Hesse MR, Deussner-Helfmann NS, Dietz MS, Kuner T, Klevanski M, Heilemann M
Frontiers in synaptic neuroscience (2021) 13: 671288. 135 307 IHC; tested species: rat
Puncta of neuronal nitric oxide synthase (nNOS) mediate NMDA-receptor signalling in the auditory midbrain.
Olthof BM, Gartside SE, Rees A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 135 307 IHC
The ZIP3 zinc transporter is localized to mossy fiber terminals and is required for kainate-induced degeneration of CA3 neurons.
Bogdanovic M, Asraf H, Gottesman N, Sekler I, Aizenman E, Hershfinkel M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 135 307 IHC-P; tested species: mouse
Cat. No.: 135 307
Amount: 200 µl
Price: $330.00
CDH2 mutation affecting N-cadherin function causes attention-deficit hyperactivity disorder in humans and mice.
Halperin D, Stavsky A, Kadir R, Drabkin M, Wormser O, Yogev Y, Dolgin V, Proskorovski-Ohayon R, Perez Y, Nudelman H, Stoler O, et al.
Nature communications (2021) 121: 6187. 135 307 ICC; tested species: mouse
Aberrant activity of mitochondrial NCLX is linked to impaired synaptic transmission and is associated with mental retardation.
Stavsky A, Stoler O, Kostic M, Katoshevsky T, Assali EA, Savic I, Amitai Y, Prokisch H, Leiz S, Daumer-Haas C, Fleidervish I, et al.
Communications biology (2021) 41: 666. 135 307 ICC; tested species: mouse
PSD-95 promotes the stabilization of young synaptic contacts.
Taft CE, Turrigiano GG
Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2014) 3691633: 20130134. 135 307 ICC; tested species: rat
Synaptic Targets of Glycinergic Neurons in Laminae I-III of the Spinal Dorsal Horn.
Miranda CO, Hegedüs K, Kis G, Antal M
International journal of molecular sciences (2023) 248: . 135 307 IHC; tested species: mouse
Visualizing Synaptic Multi-Protein Patterns of Neuronal Tissue With DNA-Assisted Single-Molecule Localization Microscopy.
Narayanasamy KK, Stojic A, Li Y, Sass S, Hesse MR, Deussner-Helfmann NS, Dietz MS, Kuner T, Klevanski M, Heilemann M
Frontiers in synaptic neuroscience (2021) 13: 671288. 135 307 IHC; tested species: rat
Puncta of neuronal nitric oxide synthase (nNOS) mediate NMDA-receptor signalling in the auditory midbrain.
Olthof BM, Gartside SE, Rees A
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) : . 135 307 IHC
The ZIP3 zinc transporter is localized to mossy fiber terminals and is required for kainate-induced degeneration of CA3 neurons.
Bogdanovic M, Asraf H, Gottesman N, Sekler I, Aizenman E, Hershfinkel M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 135 307 IHC-P; tested species: mouse
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.