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SY SY - Synaptic Systems SY SY - Synaptic Systems

VGAT
cytoplasmic domain - 131 308

GABA transporter in the membrane of synaptic vesicles

Recombinant Guinea pig purified IgG
415.00 USD
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Cat. No. 131 308 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 400 gallery  
Clone Gp117G4
Subtype IgG2 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Epitop Epitop: AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, ape.
Other species not tested yet.
Specificity Specific for mammalian VGAT. (K.O. verified)
matching control protein/peptide 131-0P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 117G4. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.

WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Datasheet 131_308.pdf


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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com