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|Cat. No. 131 308||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 400 gallery
|Subtype||IgG2 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)|
Epitop: AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Reacts with: human (Q9H598), rat (O35458), mouse (O35633), Guinea pig, ape.
Other species not tested yet.
|Specificity||Specific for mammalian VGAT. (K.O. verified)|
|matching control protein/peptide||131-0P|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 117G4. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.