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SNAP29 antibody - 111 303 K.O.

SNAP29 is a Relative of SNAP25 that distributes between the cytoplasma and organellar membranes
Rabbit polyclonal purified antibody
Cat. No.: 111 303
Amount: 50 µg
Price: $455.00
Cat. No. 111 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
IP: not tested yet
ICC: 1 : 500 (see remarks) gallery  
IHC: yes gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 1 to 257 from rat SNAP29 (UniProt Id: Q9Z2P6)
Reactivity Reacts with: human (O95721), rat (Q9Z2P6), mouse (Q9ERB0), hamster.
Other species not tested yet.
Specificity K.O. validated PubMed: 34069872
Remarks

ICC: Methanol fixation gives stronger signals.

Data sheet 111_303.pdf

References for SNAP29 - 111 303

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 111 303 WB; tested species: mouse
Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts.
Martens MC, Edelkamp J, Seebode C, Schäfer M, Stählke S, Krohn S, Jung O, Murua Escobar H, Emmert S, Boeckmann L
International journal of molecular sciences (2021) 2210: . 111 303 WB; KO verified; tested species: human
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 111 303 WB; tested species: mouse
SNAP-25 gene family members differentially support secretory vesicle fusion.
Arora S, Saarloos I, Kooistra R, van de Bospoort R, Verhage M, Toonen RF
Journal of cell science (2017) 13011: 1877-1889. 111 303 WB, ICC
Dynamics of the mouse brain cortical synaptic proteome during postnatal brain development.
Gonzalez-Lozano MA, Klemmer P, Gebuis T, Hassan C, van Nierop P, van Kesteren RE, Smit AB, Li KW
Scientific reports (2016) 6: 35456. 111 303 WB
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 111 303 WB; tested species: mouse
Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1.
Gokhale A, Larimore J, Werner E, So L, Moreno-De-Luca A, Lese-Martin C, Lupashin VV, Smith Y, Faundez V
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3211: 3697-711. 111 303 WB
SNAP-25 gene family members differentially support secretory vesicle fusion.
Arora S, Saarloos I, Kooistra R, van de Bospoort R, Verhage M, Toonen RF
Journal of cell science (2017) 13011: 1877-1889. 111 303 WB, ICC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 111 303 DNA-PAINT; tested species: rat
Cat. No.: 111 303
Amount: 50 µg
Price: $455.00
Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models.
Xie YX, Naseri NN, Fels J, Kharel P, Na Y, Lane D, Burré J, Sharma M
Nature communications (2022) 131: 4918. 111 303 WB; tested species: mouse
Generation and Characterization of a CRISPR/Cas9-Mediated SNAP29 Knockout in Human Fibroblasts.
Martens MC, Edelkamp J, Seebode C, Schäfer M, Stählke S, Krohn S, Jung O, Murua Escobar H, Emmert S, Boeckmann L
International journal of molecular sciences (2021) 2210: . 111 303 WB; KO verified; tested species: human
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 111 303 WB; tested species: mouse
SNAP-25 gene family members differentially support secretory vesicle fusion.
Arora S, Saarloos I, Kooistra R, van de Bospoort R, Verhage M, Toonen RF
Journal of cell science (2017) 13011: 1877-1889. 111 303 WB, ICC
Dynamics of the mouse brain cortical synaptic proteome during postnatal brain development.
Gonzalez-Lozano MA, Klemmer P, Gebuis T, Hassan C, van Nierop P, van Kesteren RE, Smit AB, Li KW
Scientific reports (2016) 6: 35456. 111 303 WB
The SNARE protein vti1a functions in dense-core vesicle biogenesis.
Walter AM, Kurps J, de Wit H, Schöning S, Toft-Bertelsen TL, Lauks J, Ziomkiewicz I, Weiss AN, Schulz A, Fischer von Mollard G, Verhage M, et al.
The EMBO journal (2014) 3315: 1681-97. 111 303 WB; tested species: mouse
Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1.
Gokhale A, Larimore J, Werner E, So L, Moreno-De-Luca A, Lese-Martin C, Lupashin VV, Smith Y, Faundez V
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3211: 3697-711. 111 303 WB
SNAP-25 gene family members differentially support secretory vesicle fusion.
Arora S, Saarloos I, Kooistra R, van de Bospoort R, Verhage M, Toonen RF
Journal of cell science (2017) 13011: 1877-1889. 111 303 WB, ICC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 111 303 DNA-PAINT; tested species: rat
Background

SNAP 29, also known as GS 32, is an ubiquitously distributed relative of SNAP 25 and SNAP 23 that is ubiquitously distributed among intracellular membranes and that is also found in the cytosol of mammalian cells. As a Q-SNARE it forms SNARE complexes in vitro but its precise role in intracellular membrane traffic is not known.