SNAP 25 - 111 008
Palmitoylated plasma membrane protein; required for exocytosis
Recombinant rabbit purified IgG
select country for shop
|Cat. No. 111 008||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery
IP: yes (see remarks)
ICC: 1 : 1000 gallery
IHC: not tested yet
IHC-P/FFPE: 1 : 200 up to 1 : 1000 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1)|
Epitop: AA 20 to 40 from rat SNAP25B (UniProt Id: P60881-1)
Reacts with: human (P60880), rat (P60881-1), mouse (P60879), vertebrates, invertebrates, zebrafish.
Other species not tested yet.
|Specificity||Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products. (K.O. verified)|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 71.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein.