Cat. No.: 103 011
Amount: 50 µg
Price:
$415.00
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| Cat. No. 103 011 |
50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications |
IP: yes (see remarks) ICC: not recommended IHC: not recommended IHC-P: not recommended ELISA: yes (see remarks) |
| Clone | 80.1 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Recombinant protein corresponding to AA 170 to 234 from rat Synaptogyrin1 (UniProt Id: Q62876) |
| Reactivity |
Reacts with: human (O43759), rat (Q62876), mouse (O55100), hamster. No signal: zebrafish. Other species not tested yet. |
| Specificity | Specific for synaptogyrin 1 with a weak side-reactivity towards tubulin. |
| Remarks |
IP: In immunoprecipitation (but not in other assays) tubulin coprecipitates with synaptogyrin 1 in about equal proportions. This is due to a direct binding of the antibody to tubulin. |
| Data sheet | 103_011.pdf |
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Synaptogyrins are tyrosine-phosphorylated proteins with two neuronal (synaptogyrin 1 and 3) and one ubiquitous, synaptogyrin 2 or cellugyrin isoform.
Synaptogyrins are integral membrane proteins and localize to the membrane of small vesicles. Synaptogyrin 1 and 3 are expressed in the brain whereby the latter shows a more restricted expression pattern with high levels in the mossy fiber region of the hippocampus, substantia nigra pars reticulata, pallidum, and deep cerebellar nuclei.
Synaptogyrin 2/cellugyrin, a close relative, is expressed in all tissues, for instance, in distinct populations of GLUT 4 containing vesicles.