Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

VAMP1/2/3 antibody - 104 102

Vamp1, 2 and 3 are major vesicle protein involved in fusion
Rabbit polyclonal antiserum
Cat. No.: 104 102
Amount: 200 µl
Price: $355.00
Cat. No. 104 102 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
ELISA: yes (see remarks)
Immunogen Recombinant protein corresponding to AA 1 to 82 from rat Cellubrevin (UniProt Id: P63025)
Reactivity Reacts with: human (P23763, P63027, Q15836), rat (Q63666, P63045, P63025), mouse (Q62442, P63044, P63024).
Other species not tested yet.
Specificity Specific for all three isoforms VAMP 1, 2 and 3.
Remarks

WB: This antibody detects the tetanus and Botulinum B toxin cleavage product in toxin treated tissue homogenates.
ICC: Methanol and acetone fixation; see Annaert et al., 1997
This antibody is recommended for ICC of non-neuronal cells.
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 104 211 as capture antibodies. The ELISA-protocol for membrane proteins is recommended.

Data sheet 104_102.pdf

References for VAMP1/2/3 - 104 102

Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 104 102 WB, IP; tested species: mouse
New Engineered-Botulinum Toxins Inhibit the Release of Pain-Related Mediators.
Tang M, Meng J, Wang J
International journal of molecular sciences (2019) 211: . 104 102 WB; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 104 102 WB, IP; tested species: mouse
SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity.
Kunii M, Noguchi Y, Yoshimura SI, Kanda S, Iwano T, Avriyanti E, Atik N, Sato T, Sato K, Ogawa M, Harada A, et al.
The Journal of cell biology (2021) 2201: . 104 102 ICC; tested species: mouse
Efficient transfection of dissociated mouse chromaffin cells using small-volume electroporation.
Hoerauf WW, Cazares VA, Subramani A, Stuenkel EL
Cytotechnology (2015) 673: 573-83. 104 102 ICC
Novel cell types, neurosecretory cells, and body plan of the early-diverging metazoan Trichoplax adhaerens.
Smith CL, Varoqueaux F, Kittelmann M, Azzam RN, Cooper B, Winters CA, Eitel M, Fasshauer D, Reese TS
Current biology : CB (2014) 2414: 1565-1572. 104 102 ICC
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 104 102 IHC; tested species: mouse
Cat. No.: 104 102
Amount: 200 µl
Price: $355.00
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 104 102 WB, IP; tested species: mouse
New Engineered-Botulinum Toxins Inhibit the Release of Pain-Related Mediators.
Tang M, Meng J, Wang J
International journal of molecular sciences (2019) 211: . 104 102 WB; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 104 102 WB, IP; tested species: mouse
SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity.
Kunii M, Noguchi Y, Yoshimura SI, Kanda S, Iwano T, Avriyanti E, Atik N, Sato T, Sato K, Ogawa M, Harada A, et al.
The Journal of cell biology (2021) 2201: . 104 102 ICC; tested species: mouse
Efficient transfection of dissociated mouse chromaffin cells using small-volume electroporation.
Hoerauf WW, Cazares VA, Subramani A, Stuenkel EL
Cytotechnology (2015) 673: 573-83. 104 102 ICC
Novel cell types, neurosecretory cells, and body plan of the early-diverging metazoan Trichoplax adhaerens.
Smith CL, Varoqueaux F, Kittelmann M, Azzam RN, Cooper B, Winters CA, Eitel M, Fasshauer D, Reese TS
Current biology : CB (2014) 2414: 1565-1572. 104 102 ICC
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 104 102 IHC; tested species: mouse
Background

Synaptobrevins/VAMPs represents a family of integral membrane proteins of 11-13 kDa with the N-terminal region exposed to the cytoplasm and a C-terminal transmembrane domain. Two isoforms were identified in the mammalian CNS, synaptobrevin 1 (VAMP 1 or p18-1) and synaptobrevin 2 (VAMP 2 or p18-2) that differ in their distribution within different brain regions.
Synaptobrevin 1 is highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Low levels of synaptobrevin 2 are present in many other tissues where the protein resides on specialized microvesicles.
In non-neuronal cells the third isoform, cellubrevin (VAMP 3), is present where it is localized to an endosomal membrane pool.
Synaptobrevin/VAMP is an essential component of the exocytotic fusion machine, related to a larger protein family referred to as v-SNAREs. It is the sole target for tetanus and several of the botulinal neurotoxins which cleave the protein at single sites in the C-terminal portion of the molecule.