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RIM1/2 antibody Zn-finger domain - 140 205

RIMs are Rab3 effector proteins of the cytomatrix at the active zone
Guinea pig polyclonal purified antibody
Cat. No.: 140 205
Amount: 50 µg
Price: $460.00
Cat. No. 140 205 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
ExM: 1 : 200 (see remarks) gallery  
Immunogen Recombinant protein corresponding to AA 1 to 466 from rat Rim2 (UniProt Id: Q9JIS1)
Reactivity Reacts with: rat (Q9JIS1), mouse (Q9EQZ7).
Other species not tested yet.
Specificity RIM 2 including splice variants, cross reacts to RIM 1.
Remarks

ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)

Data sheet 140_205.pdf

References for RIM1/2 - 140 205

Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus.
Lloyd BA, Han Y, Roth R, Zhang B, Aoto J
Nature communications (2023) 141: 4706. 140 205 WB; tested species: mouse
Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses.
Yang X, Le Corronc H, Legendre P, Triller A, Specht CG
EMBO reports (2021) : e52154. 140 205 ICC; tested species: rat
Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 140 205 IHC; tested species: mouse
Cat. No.: 140 205
Amount: 50 µg
Price: $460.00
Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus.
Lloyd BA, Han Y, Roth R, Zhang B, Aoto J
Nature communications (2023) 141: 4706. 140 205 WB; tested species: mouse
Differential regulation of glycinergic and GABAergic nanocolumns at mixed inhibitory synapses.
Yang X, Le Corronc H, Legendre P, Triller A, Specht CG
EMBO reports (2021) : e52154. 140 205 ICC; tested species: rat
Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1α Expressing Interneurons.
Holderith N, Aldahabi M, Nusser Z
Frontiers in synaptic neuroscience (2021) 13: 773209. 140 205 IHC; tested species: mouse
Background

RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.
RIM 2β consists of a specific N-terminus, the central PDZ domain and the C-terminal C2 domains. The mRNA for RIM 2β is transcribed from an internal promoter of the RIM 2α gene.
Shorter variants of RIM 2 which comprise only the C-terminal C2B domain and some flanking regions are referred to as NIM 2 / RIM 2γ and NIM 3 / RIM 3γ.