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Neurofilament M antibody - 171 231

Neurofilaments are major neuronal intermediate filaments
Mouse monoclonal purified IgG
Cat. No.: 171 231
Amount: 100 µg
Price: $415.00
Cat. No. 171 231 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 2000 up to 1 : 5000 gallery  
DNA-PAINT: yes (see remarks)
Clone 298A7A6
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 761 to 846 from rat Neurofilament M (UniProt Id: P12839)
Reactivity Reacts with: rat (P12839), mouse (P08553).
Other species not tested yet.
Remarks

DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).

Data sheet 171_231.pdf

References for Neurofilament M - 171 231

Inhibitory control in neuronal networks relies on the extracellular matrix integrity.
Dzyubenko E, Fleischer M, Manrique-Castano D, Borbor M, Kleinschnitz C, Faissner A, Hermann DM
Cellular and molecular life sciences : CMLS (2021) 7814: 5647-5663. 171 231 IHC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 171 231 DNA-PAINT; tested species: rat
Cat. No.: 171 231
Amount: 100 µg
Price: $415.00
Inhibitory control in neuronal networks relies on the extracellular matrix integrity.
Dzyubenko E, Fleischer M, Manrique-Castano D, Borbor M, Kleinschnitz C, Faissner A, Hermann DM
Cellular and molecular life sciences : CMLS (2021) 7814: 5647-5663. 171 231 IHC; tested species: mouse
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 171 231 DNA-PAINT; tested species: rat
Background
Neurofilaments are exclusively expressed in nerve cells and are the major structural component of large-diameter myelinated axons. They are predominatly composed of three proteins, Neurofilament H, L and M and are among the most highly phosphorylated neuronal proteins.