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GABA-A receptor alpha2 antibody extracellular - 224 104

GABA-A receptors are involved in inhibitory neurotransmission
Guinea pig polyclonal antiserum
Cat. No.: 224 104
Amount: 100 µl
Price: $365.00
Cat. No. 224 104 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 200 up to 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 29 to 37 from rat GABA-A receptor α2 (UniProt Id: P23576)
Reactivity Reacts with: human (P47869), rat (P23576), mouse (P26048).
Other species not tested yet.
Matching control protein/peptide 224-1P
Remarks

WB:  GABA-A receptor alpha2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
ICC: This antibody is also suitable for the surface staining of living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents.

Data sheet 224_104.pdf

References for GABA-A receptor alpha2 - 224 104

γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains.
Brown LE, Nicholson MW, Arama JE, Mercer A, Thomson AM, Jovanovic JN
The Journal of biological chemistry (2016) 29127: 13926-42. 224 104 ICC
Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 224 104 ICC; tested species: mouse
Loss of function of NCOR1 and NCOR2 impairs memory through a novel GABAergic hypothalamus-CA3 projection.
Zhou W, He Y, Rehman AU, Kong Y, Hong S, Ding G, Yalamanchili HK, Wan YW, Paul B, Wang C, Gong Y, et al.
Nature neuroscience (2019) : . 224 104 IHC; tested species: mouse
Activity-dependent mismatch between axo-axonic synapses and the axon initial segment controls neuronal output.
Wefelmeyer W, Cattaert D, Burrone J
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: 9757-62. 224 104 IHC
Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 224 104 UPTAKE; tested species: mouse
Cat. No.: 224 104
Amount: 100 µl
Price: $365.00
γ-Aminobutyric Acid Type A (GABAA) Receptor Subunits Play a Direct Structural Role in Synaptic Contact Formation via Their N-terminal Extracellular Domains.
Brown LE, Nicholson MW, Arama JE, Mercer A, Thomson AM, Jovanovic JN
The Journal of biological chemistry (2016) 29127: 13926-42. 224 104 ICC
Structure of excitatory synapses and GABAA receptor localization at inhibitory synapses are regulated by neuroplastin-65.
Herrera-Molina R, Sarto-Jackson I, Montenegro-Venegas C, Heine M, Smalla KH, Seidenbecher CI, Beesley PW, Gundelfinger ED, Montag D
The Journal of biological chemistry (2014) 28913: 8973-88. 224 104 ICC; tested species: mouse
Loss of function of NCOR1 and NCOR2 impairs memory through a novel GABAergic hypothalamus-CA3 projection.
Zhou W, He Y, Rehman AU, Kong Y, Hong S, Ding G, Yalamanchili HK, Wan YW, Paul B, Wang C, Gong Y, et al.
Nature neuroscience (2019) : . 224 104 IHC; tested species: mouse
Activity-dependent mismatch between axo-axonic synapses and the axon initial segment controls neuronal output.
Wefelmeyer W, Cattaert D, Burrone J
Proceedings of the National Academy of Sciences of the United States of America (2015) 11231: 9757-62. 224 104 IHC
Fast Regulation of GABAAR Diffusion Dynamics by Nogo-A Signaling.
Fricke S, Metzdorf K, Ohm M, Haak S, Heine M, Korte M, Zagrebelsky M
Cell reports (2019) 293: 671-684.e6. 224 104 UPTAKE; tested species: mouse
Background

Gamma-aminobutyric acid type A (GABA-A) receptors mediate the majority of inhibitory neurotransmission in the brain. These receptor proteins are ligand gated chloride ion channels and consist of a pentameric combination of different subunits (alpha, beta, gamma, delta, epsilon and rho). The resulting heterogenous population of GABA-A receptor subtypes are expressed throughout the brain with specific cellular and subcellular expression patterns.