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Home > Products > 250 113

EAAT1 antibody cytoplasmic domain - 250 113 K.O.

EAATs are transmembrane proteins involved in the removal of extracellular glutamate
Rabbit polyclonal purified antibody
Cat. No.: 250 113
Amount: 50 µg
Price: $375.00
Cat. No. 250 113 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 1000 up to 1 : 5000 gallery  
IHC: 1 : 1000 up to 1 : 5000 gallery  
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 522 to 541 from rat EAAT1 (UniProt Id: P24942)
Reactivity Reacts with: human (P43003), rat (P24942), mouse (P56564).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 250-11P
Data sheet 250_113.pdf

References for EAAT1 - 250 113

Spinal astrocyte dysfunction drives motor neuron loss in late-onset spinal muscular atrophy.
Schmitt LI, David C, Steffen R, Hezel S, Roos A, Schara-Schmidt U, Kleinschnitz C, Leo M, Hagenacker T
Acta neuropathologica (2023) : . 250 113 WB, IHC; tested species: mouse
Astrocyte dysfunction increases cortical dendritic excitability and promotes cranial pain in familial migraine.
Romanos J, Benke D, Pietrobon D, Zeilhofer HU, Santello M
Science advances (2020) 623: eaaz1584. 250 113 WB; tested species: mouse
Differences in glutamate uptake between cortical regions impact neuronal NMDA receptor activation.
Romanos J, Benke D, Saab AS, Zeilhofer HU, Santello M
Communications biology (2019) 2: 127. 250 113 WB; tested species: mouse
Ataxia-linked SLC1A3 mutations alter EAAT1 chloride channel activity and glial regulation of CNS function.
Wu Q, Akhter A, Pant S, Cho E, Zhu JX, Garner A, Ohyama T, Tajkhorshid E, van Meyel DJ, Ryan RM
The Journal of clinical investigation (2022) 1327: . 250 113 ICC; tested species: drosophila
Induction of Survival of Motor Neuron (SMN) Protein Deficiency in Spinal Astrocytes by Small Interfering RNA as an In Vitro Model of Spinal Muscular Atrophy.
Leo M, Schmitt LI, Fleischer M, Steffen R, Osswald C, Kleinschnitz C, Hagenacker T
Cells (2022) 113: . 250 113 ICC, IHC; tested species: mouse
Spinal astrocyte dysfunction drives motor neuron loss in late-onset spinal muscular atrophy.
Schmitt LI, David C, Steffen R, Hezel S, Roos A, Schara-Schmidt U, Kleinschnitz C, Leo M, Hagenacker T
Acta neuropathologica (2023) : . 250 113 WB, IHC; tested species: mouse
Induction of Survival of Motor Neuron (SMN) Protein Deficiency in Spinal Astrocytes by Small Interfering RNA as an In Vitro Model of Spinal Muscular Atrophy.
Leo M, Schmitt LI, Fleischer M, Steffen R, Osswald C, Kleinschnitz C, Hagenacker T
Cells (2022) 113: . 250 113 ICC, IHC; tested species: mouse
Cat. No.: 250 113
Amount: 50 µg
Price: $375.00
Spinal astrocyte dysfunction drives motor neuron loss in late-onset spinal muscular atrophy.
Schmitt LI, David C, Steffen R, Hezel S, Roos A, Schara-Schmidt U, Kleinschnitz C, Leo M, Hagenacker T
Acta neuropathologica (2023) : . 250 113 WB, IHC; tested species: mouse
Astrocyte dysfunction increases cortical dendritic excitability and promotes cranial pain in familial migraine.
Romanos J, Benke D, Pietrobon D, Zeilhofer HU, Santello M
Science advances (2020) 623: eaaz1584. 250 113 WB; tested species: mouse
Differences in glutamate uptake between cortical regions impact neuronal NMDA receptor activation.
Romanos J, Benke D, Saab AS, Zeilhofer HU, Santello M
Communications biology (2019) 2: 127. 250 113 WB; tested species: mouse
Ataxia-linked SLC1A3 mutations alter EAAT1 chloride channel activity and glial regulation of CNS function.
Wu Q, Akhter A, Pant S, Cho E, Zhu JX, Garner A, Ohyama T, Tajkhorshid E, van Meyel DJ, Ryan RM
The Journal of clinical investigation (2022) 1327: . 250 113 ICC; tested species: drosophila
Induction of Survival of Motor Neuron (SMN) Protein Deficiency in Spinal Astrocytes by Small Interfering RNA as an In Vitro Model of Spinal Muscular Atrophy.
Leo M, Schmitt LI, Fleischer M, Steffen R, Osswald C, Kleinschnitz C, Hagenacker T
Cells (2022) 113: . 250 113 ICC, IHC; tested species: mouse
Spinal astrocyte dysfunction drives motor neuron loss in late-onset spinal muscular atrophy.
Schmitt LI, David C, Steffen R, Hezel S, Roos A, Schara-Schmidt U, Kleinschnitz C, Leo M, Hagenacker T
Acta neuropathologica (2023) : . 250 113 WB, IHC; tested species: mouse
Induction of Survival of Motor Neuron (SMN) Protein Deficiency in Spinal Astrocytes by Small Interfering RNA as an In Vitro Model of Spinal Muscular Atrophy.
Leo M, Schmitt LI, Fleischer M, Steffen R, Osswald C, Kleinschnitz C, Hagenacker T
Cells (2022) 113: . 250 113 ICC, IHC; tested species: mouse
WB
ICC
IHC
Background
Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. After the release of glutamate from synaptic vesicles into the synaptic cleft during neurotransmission, excitatory amino acid transporters (EAATs) remove extracellular glutamate to avoid excitotoxic levels.
Five EAATs with differential expression patterns have been described so far: EAAT 1, also referred to as GLAST and SLC1A3, has neuroprotective potential following ischemia and is expressed by reactive astrocytes and activated microglia. EAAT 2 (GLT-1, SLC1A2) is the most abundant and primarily expressed in astrocytes. EAAT 3 / SLC1A1 is expressed in neurons and has also been shown to be involved in the uptake of extracelluar cysteine. EAAT 4 shows weak expression in the forebrain and high levels in Purkinje cells of the cerebellum. EAAT 5 has only been described for humans and is primarily expressed in the retina.
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