50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P/FFPE: not tested yet EM: yes
Synthetic peptide corresponding to AA 1778 to 1791 from rat Big2 (UniProt Id: Q7TSU1)
Reacts with: rat (Q7TSU1), mouse (A2A5R2). Other species not tested yet.
Specific for BIG 2 (K.D. verified)
IHC: Detects high levels of endogenous BIG 2 protein (e.g. in hippocampus). Regions with low expression levels may be difficult to interpret.