ZnT 3

A synaptic vesicle located zinc-transporter

Fact Sheet

Monoclonal mouse antibody
Cat. No. 197 011 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 blot IP: yes ICC: 1 : 500 image IHC: 1 : 100 image
Clone	180C1
Subtype	IgG1
Immunogen	Recombinant protein of mouse ZnT 3 (aa 2 - 75).
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for ZnT 3.

Polyclonal rabbit antibody
Cat. No. 197 002 crude antiserum	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: yes ICC: 1 : 500 image IHC: 1 : 500 (see remarks) image
Immunogen	Recombinant protein of mouse ZnT 3 (aa 2 - 75).
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for ZnT 3.
Remarks	IHC: Works also well on paraffin embedded tissue.

Polyclonal guinea pig antibody
Cat. No. 197 004 crude antiserum	100 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 500 image IHC: 1 : 500 image
Immunogen	Recombinant protein of mouse ZnT 3 (aa 2 - 75).
Reactivity	Rat, mouse; other species not tested yet.
Specificity	Specific for ZnT 3.

Control protein
Cat. No. 197-0P	100 µg lyophilized protein. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use.
Recommended dilution	Optimal concentrations should be determined by the end-user.
Remarks	This control protein consists of the recombinant protein (aa 2 - 75 of mouse ZnT 3) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.