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SY SY - Synaptic Systems SY SY - Synaptic Systems

VGLUT 2 (vesicular glutamate transporter 2, DNPI, SLC17A6)

Glutamate transporter in the membrane of synaptic vesicles

Fact Sheet Cat. No. 135 503

Polyclonal rabbit antibody against VGLUT 1/2
Cat. No. 135 503
purified antibody
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) blot
IP: not tested yet
ICC: 1 : 100
IHC: yes image
Immunogen Synthetic peptide SQPAYFEEVFGFEISK (aa 324-339 in rat VGLUT 1 and aa 332-347 in rat VGLUT 2) forced into a loop-shaped structure.
Reactivity Mammals, chicken, zebrafish.
Specificity Epitope identical in VGLUT 1 and VGLUT 2, one mismatch in VGLUT 3.
Remarks
  • This antibody is less powerful compared to those directed against the C-termini of VGLUTs but can be used for non-mammals like zebrafish.
  • VGLUTs aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples.





Synaptic Systems Gesellschaft für neurobiologische Forschung, Entwicklung und Produktion mbH | Rudolf-Wissell-Str. 28 | 37079 Goettingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com | Imprint
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com