cytoplasmic, mouse
-, rabbit
-, guinea pig
lumenal, rabbit |
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Monoclonal mouse antibody, cytoplasmic domain |
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Cat. No. 131 011 IgG, unlabeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
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Cat. No. 131 011C3 IgG, fluorescence-labeled |
100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex® (Merck Chemicals) is not compatible with Oyster dyes! |
| Applications |
WB: 1 : 500 up to 1 : 2000 (AP staining) blot
IP: yes ICC: 1 : 200 up to 1 : 1000 131 011 131 011C3 IHC: 1 : 100 up to 1 : 1000 131 011 |
| Clone | 117G4 |
| Subtype | IgG3 |
| Immunogen | Synthetic peptide AEPPVEGDIHYQR (aa 75 - 87 in rat) coupled to key-hole limpet hemocyanin via an added N- terminal cysteine. |
| Reactivity | Human, rat, mouse, guinea pig, monkey. |
| Specificity | Specific for mammalian VGAT. |
| Remarks | VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. |
Polyclonal rabbit antibody 1, cytoplasmic domain |
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Cat. No. 131 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
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Cat. No. 131 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining) 131 002
IP: yes ICC: 1 : 100 up to 1 : 1000 131 002 IHC: 1 : 500 up to 1 : 3000 131 002 |
| Immunogen | Synthetic peptide AEPPVEGDIHYQR (aa 75 - 87 in rat) coupled to key-hole limpet hemocyanin via an added N- terminal cysteine. |
| Reactivity | Human, rat, mouse, monkey; other species not tested yet. |
| Specificity | Specific for VGAT. |
| Remarks | VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. |
Control peptide for cytoplasmic domain mouse antibodies and rabbit antibodies 1 |
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| Cat. No. 131-0P |
100 µg lyophilized peptide. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. Control peptides should also be stored at -20°C when still lyophilized! |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control peptide consists of the synthetic peptide (AEPPVEGDIHYQR) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
Polyclonal rabbit antibody 2, cytoplasmic domain |
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Cat. No. 131 013 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) blot
IP: yes ICC: 1 : 500 image IHC: 1 : 500 image EM: This antibody is first choice for electron microscopy. |
| Immunogen | Strep-Tag® fusion protein of rat VGAT (aa 2 - 115). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for VGAT. |
| Remarks | VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. |
Polyclonal guinea pig antibody, cytoplasmic domain |
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Cat. No. 131 004 crude antiserum |
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 2000 (AP staining) blot
IP: not tested yet ICC: 1 : 500 up to 1 : 1000 image IHC: 1 : 200 up to 1 : 500 image |
| Immunogen | Strep-Tag® fusion protein of rat VGAT (aa 2 - 115). |
| Reactivity | Rat, mouse; other species not tested yet. |
| Specificity | Specific for VGAT. |
| Remarks | VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. |
Control protein for cytoplasmic domain guinea pig antibodies |
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| Cat. No. 131-0GP | 100 µg lyophilized peptide. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the Strep-Tag® fusion protein (aa 2 - 115) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
Polyclonal rabbit antibody, lumenal domain |
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Cat. No. 131 103 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
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Cat. No. 131 103C2 purified antibody, fluorescence-labeled |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster® 488. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex® (Merck Chemicals) is not compatible with Oyster dyes! |
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Cat. No. 131 103C3 purified antibody, fluorescence-labeled |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex® (Merck Chemicals) is not compatible with Oyster dyes! |
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Cat. No. 131 103C5 purified antibody, fluorescence-labeled |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster® 650. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex® (Merck Chemicals) is not compatible with Oyster dyes! |
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Cat. No. 131 103CpH purified antibody, CypHer-labeled |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
WB: 1 : 1000 (AP staining)
IP: yes ICC: 1 : 100 up to 1 : 200 131 103 131 103C2 131 103C5 131 103CpH IHC: yes |
| Immunogen | Synthetic peptide HSLEGLIEAYRTNAED (aa 510 - 525 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for VGAT. |
| Remarks |
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