Cat. No. 131 002 | ||
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Immunolabeling of inhibitory synapses in the central nervous system. Immunoreactivity was revealed using carbocyanine 2, 3, and 5-conjugated secondary antibodies. Neurofilaments (SMI 32) and choline-acetyltransferase (ChAT) were detected with mouse and goat antibodies. Rats were transcardially perfused with a fixative containing 4 % paraformaldehyde and 0.1 % glutaraldehyde in phosphate buffer (0.1M, pH 7.4). Courtesy: Tibor Harkany, Dept. of Medical Biochemistry & Biophysics, Karolinska Institute, Stockholm |
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