Velis (MALS, Lin 7)
Multi-adaptor-proteins participating in different complexes
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 184 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 184 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining) 184 002 184 003
IP: not tested yet ICC: 1 : 1000 184 002 IHC: not tested yet |
| Immunogen | Recombinant full length rat veli 1. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Recognizes all three isoforms veli 1, 2 and 3. |
| Control protein | |
| Cat. No. 184-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant full length rat veli 1 that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
