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Uncoating ATPase (hsc70)

A protein involved in the removal of the clathrin coat of vesicles

Fact Sheet Cat. No. 149 011

Monoclonal mouse antibody
Cat. No. 149 011
IgG, unlabeled
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 up to 1 : 5000 (AP staining) blot
IP: not tested yet
ICC: 1 : 500 image
IHC: not tested yet
Clone 3C5
Subtype IgG1
Immunogen Purified bovine ATPase.
Epitope Between aa 391-546.
Reactivity Human, rat, mouse, hamster, cow.
Specificity Specific for uncoating ATPase.
Remarks
  • For fixation a 20 min treatment with 3.7 % PFA in PBS is recommended.
  • When microinjected into cells, the antibody inhibits clathrin mediated endocytosis.





Synaptic Systems Gesellschaft für neurobiologische Forschung, Entwicklung und Produktion mbH | Rudolf-Wissell-Str. 28 | 37079 Goettingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com | Imprint
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com