α-tubulin

A major cytoskeleton protein

Fact Sheet

Remarks

Monoclonal mouse antibody against glu-α-tubulin
Glu-α-Tubulin Tyr-α-Tubulin α-Tubulin Δ2-Tubulin
Cat. No. 302 011 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 100 up to 1 : 1000 (AP staining; see remarks) blot IP: not tested yet ICC: 1 : 200 image IHC: 1 : 200 (also paraffin-embedded tissues) image ELISA: yes
Clone	1D5
Subtype	IgG1
Immunogen	Synthetic peptide VDSVEGEGEEEGEE (14 C-terminal aa of detyrosinated α-tubulin) coupled to BSA via glutaraldehyde.
Epitope	X-EE including the terminal α-carboxylate group. All linkages in the peptide involve α-peptide bonds.
Reactivity	Human, rat, mouse; eukaryotes. Detects also cilia of Paramecium.
Specificity	Specific for detyrosinated α-tubulin (glu-tubulin) and polyglutamylated tubulin (also β-tubulin). No cross reaction to tyrosinated tubulin.
WB: Tween 20 (other detergents not yet tested) should not be added to the blocking and washing solution. It greatly diminishes the intensity of the signal.

Monoclonal mouse antibody against tyr-α-tubulin
Cat. No. 302 111 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 100 up to 1 : 2000 (AP staining) blot IP: yes ICC: yes IHC: 1 : 200 image
Clone	20C6
Subtype	IgG1
Immunogen	Synthetic peptide GEEEGEEY (C-terminus of tyrosinated α-tubulin) coupled to key-hole limpet hemocyanin via glutaraldehyde.
Reactivity	Human, rat, mouse; other species not tested yet.
Specificity	Specific for tyrosinated α-tubulin (tyr-tubulin). No cross reaction to glu-α-tubulin.

Monoclonal mouse antibody against α-tubulin
Cat. No. 302 211 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Cat. No. 302 211C3 IgG, fluorescence-labeled	50 µg purified IgG, lyophilized, fluorescence-labeled with Oyster^® 550. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex^® (Merck Chemicals) is not compatible with Oyster dyes!
Cat. No. 302 201 ascites	100 µl ascites, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 50000 (AP staining) 302 201 IP: yes ICC: 1 : 500 up to 1 : 1000 302 201 302 211C3 IHC: not tested yet ELISA: yes (see remarks)
Clone	3A2
Subtype	IgG1
Immunogen	Synthetic peptide SEAREDMAALEKDYEEV (aa 419 - 435 in human α-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.
Reactivity	Human, rat, mouse; vertebrates, invertebrates; yeast.
Specificity	Specific for α-tubulin (glu- and tyr-α-tubulin).
Remarks	ELISA: Suitable as capture antibodies for sandwich-ELISA with cat. no. 302 203 as detector antibody (protocol for sandwich-ELISA).

Polyclonal rabbit antibody against α-tubulin
Cat. No. 302 203 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 5000 (AP staining) IP: not tested yet ICC: 1 : 100 up to 1 : 1000 image IHC: not tested yet ELISA: yes (see remarks)
Immunogen	Synthetic peptide EGEEEGE (aa 443 - 449 of rat α-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
Reactivity	Human, rat, mouse; mammals; chicken.
Specificity	Specific for α-tubulin (glu- and tyr-α-tubulin).
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 302 211 or 302 201 as capture antibodies (protocol for sandwich-ELISA).

Monoclonal rat antibody against α-tubulin
Cat. No. 302 217 IgG, unlabeled	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining; see remarks) blot IP: not tested yet ICC: 1 : 200 up to 1 : 500 image IHC: 1 : 400 image
Clone	37B5
Subtype	IgG1
Immunogen	Synthetic peptide EGEEEGE (aa 443 - 449 of rat α-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.
Reactivity	Mammals, chicken.
Specificity	Specific for α-tubulin.

Polyclonal rabbit antibody against Δ2-tubulin
Cat. No. 302 213 purified antibody	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use.
Applications	WB: 1 : 1000 (AP staining) blot IP: not tested yet ICC: 1 : 200 (see remarks) IHC: not tested yet
Immunogen	Synthetic peptide EGEEEGE (aa 443 - 449 of rat α-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.
Reactivity	Human, rat, mouse; mammals.
Specificity	Specific for Δ2-tubulin.
Remarks	This antibody has been cross-adsorbed against peptides corresponding to the C-termini of glu- and tyr-tubulin. ICC: Requires long permeabilization with 0.3% Tritonx100 for 30 min.