| Monoclonal rat antibody against α-tubulin, clone 37B5 | |
|---|---|
|
Cat. No. 302 217 IgG, unlabeled |
100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining; see remarks) blot
IP: not tested yet ICC: 1 : 200 up to 1 : 500 image IHC: 1 : 400 image |
| Clone | 37B5 |
| Subtype | IgG1 |
| Immunogen | Synthetic peptide EGEEEGE (aa 443 - 449 of rat α-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue. |
| Reactivity | Mammals, chicken. |
| Specificity | Specific for α-tubulin. |