Tissue lysates

General Information for Brain Lysates

In many cases the relative concentration of the protein of interest is high enough to use whole brain lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. Four fractions have been prepared according to our protocol. Protein concentration has been determined with a Bradford protein quantification assay.