Tissue lysates
Fact Sheet
| Brain lysates | |
|---|---|
|
Cat. No. 501-TOT whole brain |
200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
|
Cat. No. 502-P2 synaptosomal fraction |
100 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 50 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
|
Cat. No. 503-LP1 synaptic membrane fraction |
100 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 50 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
|
Cat. No. 504-LP2 synaptic vesicle fraction |
50 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 50 µl. Concentration: 1 mg/ml. Aliquot and store at -20°C until use. |
| Loading/lane | 10 µg blot |
| Source | adult Wistar rat |
| Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol. | |
| Spinal cord lysates | |
|
Cat. No. 505-MSC whole spinal cord |
200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
| Loading/lane | 10 µg blot |
| Source | adult Balb/c mouse |
| Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol. | |
| Other tissue lysates | |
|
Cat. No. 601-SPL whole spleen homogenate |
200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
|
Cat. No. 602-KID whole kidney homogenate |
200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
|
Cat. No. 603-HEA whole heart homogenate |
200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
|
Cat. No. 604-LIV whole liver homogenate |
200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
|
Cat. No. 605-LUN whole lung homogenate |
200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
|
Cat. No. 606-GUT whole gut homogenate |
200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 100 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use. |
| Loading/lane | 10 µg blot |
| Source | adult Wistar rat |
| Since several proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol. | |
