| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 314 002 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 314 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 (AP staining) 314 003
IP: not tested yet ICC: 1 : 1000 314 002 314 003 IHC: not tested yet |
| Immunogen | Recombinant mouse tau (aa 3 - 214). |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for tau. |
| Remarks | The antibody binds phosphorylated and non-phosphorylated tau proteins. |
| Control protein | |
| Cat. No. 314-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein (aa 3 - 214 of mouse tau) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |