Syntenin
An ubiquitously expressed PDZ domain protein
Fact Sheet
| Monoclonal mouse antibody | |
|---|---|
|
Cat. No. 133 011 IgG, unlabeled |
100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 100 (ECL detection, see remarks) blot
IP: not tested yet ICC: 1 : 100 up to 1 : 500 image IHC: not tested yet |
| Clone | 32A6 |
| Subtype | IgG1 |
| Immunogen | Recombinant full-length human syntenin. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Recognizes syntenin 1 and 2. |
| Remarks | Sensitive detection methods like ECL are required to detect endogenous syntenin by WB. |
| Polyclonal rabbit antibody | |
|
Cat. No. 133 003 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 100 up to 1 : 1000 (AP staining) blot
IP: yes ICC: yes IHC: 1 : 1000 image |
| Immunogen | Recombinant full-length human syntenin. |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity |
|
| Control protein for rabbit antibody | |
| Cat. No. 133-0P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution of protein in citric acid, pH 3. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant full-length human syntenin that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
