Syntaxin 7
A syntaxin family member functioning in endosomal and lysosomal fusion reactions
Fact Sheet
| Polyclonal rabbit antibody | |
|---|---|
|
Cat. No. 110 072 crude antiserum |
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use. |
|
Cat. No. 110 073 purified antibody |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications |
WB: 1 : 1000 up to 1 : 5000 (AP staining) 110 072 110 073
IP: yes (only crude serum tested) ICC: 1 : 100 (only crude serum tested) IHC: not tested yet |
| Immunogen | Recombinant cytoplasmic domain of rat syntaxin 7 (aa 1 - 236). |
| Reactivity | Human, rat, mouse; other species not tested yet. |
| Specificity | Specific for syntaxin 7. |
| Control protein | |
| Cat. No. 110-7P |
100 µg lyophilized protein. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C until use. |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Remarks | This control protein consists of the recombinant protein (aa 1 - 236) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
